Infectious Diseases
Infectious disease research covers a broad range of topics including microbial pathogenesis, epidemiology, immunology, and antimicrobial drug and vaccine development. Promega supplies many basic molecular biology reagents that are used extensively by researchers studying these disease processes. These products include genomic DNA, plasmid DNA and RNA extraction kits, sequencing reagents, cell health assays, cloning enzymes, and PCR systems.
In addition to these routine molecular tools, luminescence technologies such as firefly and NanoLuc® luciferase reporters and HiBiT protein tagging and detection system have found extensive application in the study of viral diseases. The small size of the Nanoluc® luciferase reporter and HiBiT tag make them particularly useful in small viral genomes, and the bright luminescence signal generated makes it possible to gain sensitivity in detecting viral activity in cells and organisms.
Viral Research, Testing and Assay Development
Infectious Disease Product Categories
Cell Health Assays
Ensayos para medir la apoptosis, la viabilidad, la citotoxicidad y la inflamación.
Cloning and DNA Markers
Enzimas de restricción, vectores para clonaje, marcadores de peso molecular y células competentes.
Energy Metabolism
Ensayos de actividad metabólica, incluida la captación de glucosa, el estrés oxidativo y la detección de dinucleótidos.
Immunoassays
Ensayos Lumit® para una rápida inmunodetección bioluminiscente, así como anticuerpos primarios y secundarios y reactivos para blotting.
Luciferase Assays
Ensayos y vectores reporteros, líneas celulares que expresan luciferasa y reactivos de transfección.
Nucleic Acid Extraction
Kits de extracción adaptables que generan altos rendimientos para muchas muestras y niveles de procesamiento.
PCR
Productos y soluciones de alta calidad para amplificación
Protein Interactions
BRET, HaloTag y ensayos de interacción y pulldown de proteínas basados en GST.
Sequencing
Productos y soluciones para obtener muestras de alta calidad para NGS o pruebas de confirmación.
Example Infectious Disease Applications
Virology
Luciferases are increasingly being used to study viral infectious diseases. These bioluminescence-based methods provide highly sensitive detection of specific cellular events such as viral entry, proliferation, and viral:host protein interactions.
Cellular Immune Response
Promega cell health and energy metabolism products are applied to study many aspects of the host response to pathogens, including inflammasome activation, host cell metabolic changes and host cell viability.
