Live-Cell Kinetic Assays for Time Course Analysis
Are you performing time-course and dose-response experiments?
Are you working with precious primary or patient cells?
Are you doing drug screenings?
Live-cell kinetic assays are detection reagents that allow the same sample well to be repeatedly measured over multiple time points. This saves you time and effort, enabling you to collect more informative data in real time. Learn how you can get better quantitative data without cumbersome multi-plate setups.
Webinar: Real-Time Cell Health Assays Reveal More Relevant Biology
Discover a new generation of sensitive, microtiter plate-based, live cell assays that allow the continuous measurement of different biomarkers of cell health and apoptosis.
Live-Cell Kinetic Assays and Citations
We offer a portfolio of individual live-cell kinetic assay reagent kits that measure a variety of cellular responses. Learn more about what you can measure from the table below, and search Google Scholar for peer-reviewed publications using our assays.
| What You Can Measure | Molecular Readout | Kinetic Assay Kit | Peer-Reviewed Citations |
|---|---|---|---|
| Apoptosis and Necrosis | Annexin V: Phosphatidylserine binding | RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay | Search Citations |
| Cell Viability | Reducing potential of cells | RealTime-Glo™ MT Cell Viability Assay | Search Citations |
| Cytotoxicity | Membrane impermeable DNA dye | CellTox™ Green Cytotoxicity Assay | Search Citations |
| Cytotoxicity | LDH Release | LDH-Glo™ Cytotoxicity Assay | Search Citations |
| Metabolic Phenotype (including glycolysis and glutaminolysis) | Glucose | Glucose-Glo™ Assay | Search Citations |
| Metabolic phenotype, including glycolysis and glutaminolysis | Glutamine and glutamate | Glutamine/Glutamate-Glo™ Assay | Search Citations |
| Metabolic phenotype, including glycolysis and glutaminolysis | Glutamate | Glutamate-Glo™ Assay | Search Citations |
| Metabolic phenotype, including glycolysis and glutaminolysis | Lactate | Lactate-Glo™ Assay | Search Citations |
| Protein:Protein Interactions | Various | NanoBiT® PPI Starter Systems | Search Citations |
| Reporter Expression | Various | Nano-Glo™ Extended Live Cell Substrates | Search Citations |
| Target Engagement | Various (including epigenetic and kinases targets) | NanoBRET® Target Engagement Assays | Search Citations |
Want to visualize rare proteins and cellular events in real-time?
Discover the future of bioluminescence microscopy—the GloMax® Galaxy Bioluminescence Imager.
Transform Your Time-Course into a One-Plate Experiment
No more separate wells for every time point. One plate setup gets you unlimited data.
Less setup, less cells, less time, less pipetting, less reagents, less... well... everything.
Standard Apoptosis Multi-Plate Method
Real-Time Apoptosis Assay
Download this poster to learn how the RealTime-Glo™ Annexin V Apoptosis Assay can be used with various cell models exploring the kinetics of apoptosis.
Learn How These Assays Work
We provide a portfolio of individual live-cell kinetic assay kits. All of these assays allow you to read the same sample plate over multiple time points. The workflow varies based on the different reagent chemistry.
Example Workflow #1: Repeated Reading from Same Well
Plate your cells and add the reagent once. Then read the plate at each time point, as many times as you need! Our Cell Viability, Cytotoxicity, Target Engagement, Reporter Expression, Apoptosis, Extracellular ATP, Protein:Protein Interactions and Targeted Protein Degradation assays use this workflow.
Example Workflow #2: Repeated Sampling from Same Well
Plate your cells just once. At each time point, remove a small sample of cell media, add reagent and read. Repeat sampling as many times as you need! Our LDH Release and Energy Metabolism assays use this workflow.
See how our RealTime-Glo™ MT Cell Viability Assay works
Learn how the RealTime-Glo™ MT Cell Viability Assay is used to screen for novel cancer drugs
No More Missing Time Points
Every second, cellular conditions are changing.
What if you miss that one time point that matters?
With real-time assays, you don't need to guess when things will be interesting.
Make a real curve! Collect data from however many time points you need, all from a single well.
Data obtained using the Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates.
