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Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

ADP-Glo™ Kinase Assay

V9101_ADP-Glo--Kinase-Assay--1000-Assays_3
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One Assay for All Your Kinase Screening Needs

  • Use with virtually any kinase
  • Stable luminescent signal
  • Sensitive to low concentrations of ADP

Size

Catalog number selected: V6930

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This product is discontinued
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ADP-Glo™ Kinase Assay
400 assays
Your price:
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A Luminescent ADP Detection System for Kinases and Other ADP-Generating Enzymes

The ADP-Glo™ Kinase Assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.

The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling. Z′-factors >0.7 are routinely obtained. The stable luminescent signal allows batch plate processing without the need for strictly timed incubations.

Simple Kinase Assay Protocol

8051MB-Web-Version
 

Detects Activity of a Range of Kinases

The ADP-Glo™ Kinase assay has a high dynamic range and produces a strong signal at low ATP-to-ADP conversion, making it well suited for screening low-activity kinases such as growth factor receptor tyrosine kinases. The assay can be used at ATP concentrations up to 1mM, important for kinases with high Km values for ATP.

9171MB-W
9168MB-W
9169MB-W
9170MB-W

Use ADP-Glo™ Kinase Assay with Kinase Enzyme Systems to Simplify and Speed Kinase Profiling

Kinase Selectivity Profiling Systems

The Kinase Selectivity Profiling Systems provide kinase and substrate pairs grouped in single kinase families or as a general panel representative of the human kinome.

Kinase Profiling System Cat #
Kinase Selectivity Profiling System: CMGC-1, CMGC-2
V6854, V6856
Kinase Selectivity Profiling System:  AGC-1, AGC-2
V6858, V6910
Kinase Selectivity Profiling System:  CAMK1, CAMK2
V6932, V6924
Kinase Selectivity Profiling System:  TKL-1, STE-1
V6914, V6916
Kinase Selectivity Profiling System:  Other/CK-1
V6914, V6916
Kinase Selectivity Profiling System:  General Panel
V6928, V6929
Kinase Enzyme Systems
18401-WB-GN-Kinome

Each Kinase Enzyme System provides an individual kinase, substrate, and reaction buffer with optional ADP-Glo™ reagents. Kinase enzyme systems span the breadth of the human kinome; assays for quantifying the activity of over 170 kinases are available. 

Lipid Kinase Assay Reagents

Promega lipid kinase enzymes, substrates and detection systems provide a complete set of reagents for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The reagents include purified human recombinant proteins of Class I PI3Ks, optimized reaction buffer and ready-to-use lipid kinase substrates. 

Lipid Kinase Assays Cat.#
ADP-Glo™ Kinase Assay with Lipid Kinase Substrates
V1781, V1791
PI3K Lipid Kinases
V1791

Kinase profiling is crucial for developing targeted kinase inhibitors. The ADP-Glo™ Assay, used with the Kinase Profiling Systems, Kinase Enzyme Systems or Lipid Kinase Assay Reagents, provides a convenient, simple method for profiling the effect of lead compounds on kinase activity.

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

Explore Now

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: V6930 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Reagent

 V912C 1 × 2ml

Ultra Pure ATP, 10mM

 V915A 1 × 500μl

ADP, 10mM

 V916A 1 × 500μl

Kinase Detection Reagent

 V917A 1 × 4ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V9101 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Reagent

 V912A 1 × 5ml

Kinase Detection Buffer

 V913A 1 × 10ml

Kinase Detection Substrate

 V914A 1 × 1 each

Ultra Pure ATP, 10mM

 V915A 1 × 500μl

ADP, 10mM

 V916A 1 × 500μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V9102 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Reagent

 V912B 1 × 50ml

Kinase Detection Buffer

 V913B 1 × 100ml

Kinase Detection Substrate

 V914B 1 × 1 each

Ultra Pure ATP, 10mM

 V915B 1 × 5ml

ADP, 10mM

 V916B 1 × 5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V9103 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

 V9102 10 × 10,000 assays

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V9104 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Reagent

 V912B 10 × 50ml

Kinase Detection Buffer

 V913B 10 × 100ml

Kinase Detection Substrate

 V914B 10 × 1 each

Ultra Pure ATP, 10mM

 V915B 10 × 5ml

ADP, 10mM

 V916B 10 × 5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Resources

Articles

Citations

Posters

Other Resources

Similar Products

Kinase-Glo® Luminescent Kinase Assays

Determines activity of purified kinases by quantifying ATP remaining after a kinase reaction.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774

ADP-Glo™ Max Assay

Luminescent detection of ATPase or kinase activity.

V7001, V7002

Frequently Used With

GloMax® Explorer System

Easy-to-use, flexible multimode detection system.

GM3500, GM3510

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

GloMax® Navigator System

Dedicated microplate luminometer.

GM2000, GM2010

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