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ADP-Glo™ Kinase Assay with Lipid Kinase Substrates

V9101_ADP-Glo--Kinase-Assay--1000-Assays_3
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Substrate and Assay Reagents for Detecting Class I PI3K Activity

  • Use in screening assays with purified PI3K Class I lipid kinases
  • Simple, scalable "add-and-read" assay format

Choose a substrate

Size

Catalog number selected: V1781

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ADP-Glo™ Kinase Assay with Lipid Kinase Substrates
PI:3PS/1,000 assays
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Change Configuration

Ready-to-Use Lipid Kinase Substrates and Detection Reagents

The ADP-Glo™ Kinase Assay with PI:3PS (Cat.# V1781, V1782) contains PI:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

The ADP-Glo™ Kinase Assay with PIP2:3PS (Cat.# V1791, V1792) contains PIP2:3PS Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

These products are designed for use in screening assays with purified PI3K Class I lipid kinases. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.

The ADP-Glo™ Kinase Assay with PI:3PS and the ADP-Glo™ Kinase Assay with PIP2:3PS  are components of the PI3K-Glo™ Class I Profiling Kit (Cat.# V1690).

Lipid Kinase Assay Principle

The lipid kinase reaction is performed by incubating lipid substrate (PI:3PS or PIP2:3PS) with a recombinant enzyme and ATP,  and kinase activity is measured using the ADP-Glo™ Kinase Assay. After the kinase reaction, an ATP-depletion reagent is added to terminate the lipid kinase reaction and deplete any remaining ATP, leaving only ADP. Next, a detection reagent is added to simultaneously convert ADP to ATP, which is converted to a luminescent signal using a coupled luciferase/luciferin reaction. 

Principle of the ADP-Glo™ Lipid Kinase Assay.

Protocols

Complete Protocol

Specifications

You are viewing: V1781 Change Configuration

What's in the box?

Item Part # Size Available Separately
PI:3PS Substrate, 1mg/ml PI V171A 1 × 0.5ml View Product
Lipid Dilution Buffer, 10X V180A 1 × 1ml

MgCl2, 1M

 V330A 1 × 1ml

ADP-Glo™ Reagent

 V912A 1 × 5ml

Kinase Detection Buffer

 V913A 1 × 10ml

Kinase Detection Substrate

 V914A 1 × 1 each

Ultra Pure ATP, 10mM

 V915A 1 × 500μl

ADP, 10mM

 V916A 1 × 500μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Technical Manual for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V1782 Change Configuration

What's in the box?

Item Part # Size Available Separately

ADP-Glo™ Reagent

 V912B 1 × 50ml

Kinase Detection Buffer

 V913B 1 × 100ml

Kinase Detection Substrate

 V914B 1 × 1 each

Ultra Pure ATP, 10mM

 V915B 1 × 5ml

ADP, 10mM

 V916B 1 × 5ml
PI:3PS Substrate, 1mg/ml PI V171A 10 × 0.5ml View Product
Lipid Dilution Buffer, 10X V180A 5 × 1ml

MgCl2, 1M

 V330A 1 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Technical Manual for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V1791 Change Configuration

What's in the box?

Item Part # Size Available Separately
PIP2:3PS Substrate, 1mg/ml PIP2 V170A 1 × 0.25ml View Product
Lipid Dilution Buffer, 10X V180A 1 × 1ml

MgCl2, 1M

 V330A 1 × 1ml

ADP-Glo™ Reagent

 V912A 1 × 5ml

Kinase Detection Buffer

 V913A 1 × 10ml

Kinase Detection Substrate

 V914A 1 × 1 each

Ultra Pure ATP, 10mM

 V915A 1 × 500μl

ADP, 10mM

 V916A 1 × 500μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Technical Manual for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Specifications

You are viewing: V1792 Change Configuration

What's in the box?

Item Part # Size Available Separately

ADP-Glo™ Reagent

 V912B 1 × 50ml

Kinase Detection Buffer

 V913B 1 × 100ml

Kinase Detection Substrate

 V914B 1 × 1 each

Ultra Pure ATP, 10mM

 V915B 1 × 5ml

ADP, 10mM

 V916B 1 × 5ml
PIP2:3PS Substrate, 1mg/ml PIP2 V170A 10 × 0.25ml View Product
Lipid Dilution Buffer, 10X V180A 5 × 1ml

MgCl2, 1M

 V330A 1 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Technical Manual for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Similar Products

PI3K-Glo™ Class I Profiling Kit

Detects class I PI3K activities. Includes kinases, substrates and detection reagents.

V1690

ADP-Glo™ Max Assay

Luminescent detection of ATPase or kinase activity.

V7001, V7002

ADP-Glo™ Kinase Assay

Universal, luminescent kinase activity assay.

V6930, V9101, V9102, V9103, V9104

Lipid Kinase Substrates

Used in screening assays with purified PI3K Class I lipid kinases and ADP-Glo™ Kinase Assay

V1701, V1711

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