Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

If you are located in the EEAEuropean Economic Area, the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookies in the footer of our website.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Kinase Selectivity Profiling Systems—General Panel

V6928-12355MA-panelO
V6928-12355MA-panelB
V6928-12355MA-panelC
V6929_Kinase-Selectivity-Profiling-General-Panel
V6928-12355MA-panelO
customize-this-small

Selectivity Screening of Library Compounds for Effect on Panels of Kinases

  • 24 kinase and substrate pairs organized in three 8-tube strips
  • Simple reaction assembly and automation-friendly protocol
  • Streamlines profiling of hits and leads for potency against families of related kinases

Choose a kinase profiling system

Additional options

Catalog number selected: V6928

Please Enquire
This product is discontinued
Request a Sample
Not available in all countries
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Kinase Selectivity Profiling Systems—General Panel
General Panel
Your price:
Change Configuration

Profile Lead Compounds in Hours, Not Days

The Kinase Selectivity Profiling Systems are ideal for profiling lead-compound kinase inhibitors against a broad panel of kinases to better understand inhibitor activity and obviate any off-target effects. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

The General Panel Kinase Selectivity Profiling System contains 24 kinases arranged in three 8-well strips, the corresponding substrates and cofactors, and kinase reaction buffer.

Optimized for Use With ADP-Glo™ Kinase Assay

The Kinase Selectivity Profiling Systems rely on the luminescent ADP-Glo™ Kinase Assay technology to characterize kinase selectivity. The luminescence generated by the ADP-Glo™ Kinase Assay is proportional to ADP concentration and correlates with kinase activity.

Each Kinase Selectivity Profiling System is available with or without ADP-Glo™ Assay reagents.

32460619-starred-callout

Simple, Automation-Friendly Assay, No Time-Consuming Optimization

Simply dilute the stock solutions provided, and you’re ready to go.  Two quick, one-step dilutions provide working stocks of kinases and substrate/cofactor solutions. 

Concentrated kinases and substrates/cofactor stocks are first diluted and then combined with test compound in a 384-well plate. After a 1-hour incubation, the ADP-Glo™ Assay is performed to measure kinase activity.

Kinase profiling method overview 12059ma-w

Assays can be performed manually, or automated on a liquid-handler.

Single-Dose Profiling

Each Kinase Selectivity Profiling System provides enough material to profile up to 20 compounds at a single dose or create a dose response for two compounds against eight kinases at once. 
12060MB-W
Example single-dose inhibitor profiles of eight compounds created using the Kinase Selectivity Profiling System—TK-3. Kinase assays were assembled in the presence of the indicated compounds (1μM final concentration). Kinase activity was quantified using the ADP-Glo™ Kinase Assay. The percent activity of each kinase in the presence of each compound is shown.

Custom Kinase Assay Strips Available

If you need a different combination of kinases than those provided in our catalog, you can design your own custom profiling system using our kinase selector tool.

Protocols

Complete Protocol

Specifications

You are viewing: V6928 Change Configuration

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V6929 Change Configuration

What's in the box?

Item Part # Size

Kinase Selectivity Profiling System: General Panel

 V6928 1 × 24 × 50 reactions

ADP-Glo™ Kinase Assay

 V6930 3 × 400 assays

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

Resources

Other Resources

Similar Products

Kinase Selectivity Profiling Systems—AGC

AGC family kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6858, V6859, V6910, V6931

Kinase Selectivity Profiling Systems—CAMK1 and CAMK2

Calcium/calmodulin-dependent kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6932, V6913, V6924, V6925

Kinase Selectivity Profiling Systems—CMGC

CMGC kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6854, V6855, V6856, V6857

Kinase Selectivity Profiling Systems—TK1, TK2, TK3, TK4

Tyrosine kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6850, V6851, V6852, V6853, V6920, V6921, V6922, V6923

Kinase Selectivity Profiling Systems—STE and TKL

STE and TKL kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6916, V6917, V6914, V6915

Kinase Selectivity Profiling Systems—Other

Selected kinases and substrates in an 8-tube strip format optimized for kinase profiling.

V6918, V6919, V6926, V6927

Frequently Used With

ADP-Glo™ Kinase Assay

Universal, luminescent kinase activity assay.

V6930, V9101, V9102, V9103, V9104

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

GloMax® Navigator System

Dedicated microplate luminometer.

GM2000, GM2010

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.