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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Riboprobe® Systems

P1420_Riboprobe--System---SP6_3
P1430_Riboprobe--System---T3_3
P1440_Riboprobe--System---T7_3
P1420_Riboprobe--System---SP6_3
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Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts

  • Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase
  • SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
  • Allow synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct

Choose a system

Catalog number selected: P1420

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Riboprobe® Systems
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The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.

Applications of the Riboprobe® Systems include:

  • Generation of high-specific-activity RNA probes.
  • Generation of microgram quantities of RNA transcripts.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

Protocols

Complete Protocol

Specifications

You are viewing: P1420 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

RQ1 RNase-Free DNase

 M610C 1 × 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

 N251A 1 × 2,500u 40u/μl View Product

SP6 RNA Polymerase

 P108E 1 × 500u View Product

rATP

 P113A 1 × 50μl 10mM View Product

rCTP

 P114A 1 × 50μl 10mM View Product

rGTP

 P115A 1 × 50μl 10mM View Product

rUTP

 P116A 1 × 50μl 10mM View Product

DTT

 P117A 1 × 100μl 100mM View Product

Transcription Optimized 5X Buffer

 P118B 1 × 500μl View Product

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

pGEM® Express Positive Control Template

 P256A 1 × 5μg View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Specifications

You are viewing: P1430 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

RQ1 RNase-Free DNase

 M610C 1 × 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

 N251A 1 × 2,500u 40u/μl View Product

rCTP

 P114A 1 × 50μl 10mM View Product

rATP

 P113A 1 × 50μl 10mM View Product

rGTP

 P115A 1 × 50μl 10mM View Product

rUTP

 P116A 1 × 50μl 10mM View Product

DTT

 P117A 1 × 100μl 100mM View Product

Transcription Optimized 5X Buffer

 P118B 1 × 500μl View Product

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

T3 RNA Polymerase

 P208A 1 × 500u View Product

pGEM® Express Positive Control Template

 P256A 1 × 5μg View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Specifications

You are viewing: P1440 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

RQ1 RNase-Free DNase

 M610C 1 × 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

 N251A 1 × 2,500u 40u/μl View Product

rATP

 P113A 1 × 50μl 10mM View Product

rCTP

 P114A 1 × 50μl 10mM View Product

rGTP

 P115A 1 × 50μl 10mM View Product

rUTP

 P116A 1 × 50μl 10mM View Product

DTT

 P117A 1 × 100μl 100mM View Product

Transcription Optimized 5X Buffer

 P118B 1 × 500μl View Product

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

T7 RNA Polymerase

 P207D 1 × 500u View Product

pGEM® Express Positive Control Template

 P256A 1 × 5μg View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Resources

Articles

Citations

Similar Products

Riboprobe® Combination Systems

Generate high-specific-activity RNA probes or transcripts; choice of SP6/T7 or T3/T7 systems.

P1450, P1460

Riboprobe® System Components and Buffers

Package of Transcription-Optimized Buffer, rNTPs and Nuclease-Free Water.

P1121

RiboMAX™ Large-Scale RNA Production Systems

Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.

P1280, P1300

T7 RiboMAX™ Express Large-Scale RNA Production System

Designed for speed. Produces 5–8.5mg/ml RNA in 30 minutes.

P1320

Ribo m7G Cap Analog

Enhances translation efficiency in many reticulocyte-based reactions.

P1711, P1712

Frequently Used With

pGEM® Express Positive Control Template

Used to monitor in vitro transcription reactions with the Riboprobe® Systems.

P2561

PureYield™ Plasmid Midiprep System

Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.

A2492, A2495, A2496

rATP, rCTP, rUTP, rGTP

Set of four rNTPs supplied at 10mM or 100mM.

P1221, E6000

T7 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription that has high specificity for T7 promoter sequences.

P2075, P2077, P4074, P618X, P617X

Let's find the product that meets your needs.

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Chris

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