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Maxwell® RSC Fecal Microbiome DNA Kit

AS1700_Maxwell-RSC-Fecal-Microbiome-DNA-Purification-Kit_3
customize-this-small

Reliable, Automated DNA Purification from Fecal Samples

  • Purifies high-quality DNA from ≤300mg of feces
  • Ready for use in PCR, qPCR, 16S metagenomic sequencing
  • No need for labor-intensive sample processing

Size

Catalog number selected: AS1700

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Maxwell® RSC Fecal Microbiome DNA Kit
48 preps
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Simple, Reproducible DNA Extraction from Fecal Samples

The Maxwell® RSC Fecal Microbiome DNA Kit used with the Maxwell® RSC Instruments provides a simple method for efficient, automated isolation of nucleic acid from human and mammalian fecal samples.

Fecal microbiome characterization is a rapidly evolving field, with continued development of downstream assays. Complex analyses such as next-generation sequencing can enhance data quality by measuring the abundance of various bacterial species. Many extraction methods for fecal microbiome DNA involve several manual processing steps and mechanical disruption. The Maxwell® RSC Fecal Microbiome DNA Kit purifies microbiome DNA for characterization in downstream assays including qPCR and next-generation sequencing.

Advantages

  • Simple, automated protocol requires minimal hands-on steps.
  • No organic extractions or laborious sample preprocessing steps.
  • Optimized to extract microbial DNA from fecal samples for metagenomic analysis.
Quick protocol for fecal DNA extraction

A quick protocol for fecal DNA extraction without the need for bead beating.

Yield and Concentration of DNA from Fecal Samples

Yield and Concentration of DNA extracted from feces

DNA from 100mg and 300mg of human fecal samples was extracted using the Maxwell® RSC Fecal Microbiome DNA Kit. Yield and concentration of extracted DNA was determined using the QuantiFluor® dsDNA System (Cat.# E2670, E2671).

Maxwell® Fecal Microbiome Kit: Compatibility with Stool Collection Devices

We continue to test the performance of the Maxwell® RSC Fecal Microbiome DNA Purification Kit for isolation of DNA under various conditions. View the application notes below to see protocols and example NGS data demonstrating use of this kit with different collection devices, including Copan FecalSwab® DNA/RNA Shield, OMNIgene• GUT and S.T. A. R. Buffer.

View Application Notes
DNA Extraction Protocols image-56882596

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Maxwell® RSC Cartridge (RSCJ)

 AS159A 48 × 1 each

Proteinase K (PK) Solution

 MC500C 2 × 1ml View Product

Lysis Buffer

 MC141A 1 × 100ml View Product

RNase A Solution

 A797C 1 × 1ml 4mg/ml View Product

Elution Buffer

 A828D 1 × 20ml View Product

Binding Buffer

 MC501D 1 × 20ml

RSC Plungers

 AS1670 1 × 48/pack View Product

Elution Tubes (0.5ml)

 AS620A 1 × 50/pk View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

Software and Firmware

Other Resources

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AS1400, ASB1400

Maxwell® RSC ccfDNA Plasma Kit

Extracts circulating cell-free DNA from plasma or serum using the Maxwell® RSC Instrument.

AS1480, AS1840

Maxwell® RSC Viral Total Nucleic Acid Purification Kit

Viral total nucleic acid extraction from various samples types using the Maxwell® RSC Instrument.

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Frequently Used With

Maxwell® RSC Instrument

Benchtop instrument for automated nucleic acid purification with integrated quantitation. For Research Use Only.

AS4500

Quantus™ Fluorometer and NGS Starter Package

Two fluorescence channels for nucleic acid and protein quantitation. Available in bundle for NGS applications.

E6150, E5150

QuantiFluor® dsDNA System

Specific, sensitive dsDNA quantitation system.

E2670, E2671

Maxwell® RSC 48 Instrument

Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.

AS8500

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