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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Maxwell® RSC Plant DNA Kit

Maxwell RSC Plant DNA Kit 48 preps
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Automated DNA Extraction from Plant Tissues

  • Extracts DNA from a range of plant tissues, including soybean, corn and Arabidopsis
  • Consistent purification, no organic extractions and minimal preprocessing
  • Purified DNA is ready to use in downstream applications including amplification assays

Size

Catalog number selected: AS1490

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Maxwell® RSC Plant DNA Kit
48 preps
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Automated DNA Extraction from Plant Tissue

The Maxwell® RSC Plant DNA Kit is used with the Maxwell® RSC and RSC 48 Instruments to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from plant tissue samples. The Maxwell® RSC Instrument can process from 1 to 16 samples, and the Maxwell® RSC 48 can process from 1 to 48 samples in a single run. 

Using this system, DNA can be purified from plant samples in under 60 minutes with minimal preprocessing and no organic extractions. Automated purification results in consistent purification with less variability than traditional DNA extraction methods such as CTAB and spin-columns.

The Instruments are supplied with preprogrammed purification methods and use predispensed reagent cartridges, maximizing simplicity and convenience.

Purified DNA Shows Minimal Inhibition in Downstream Assays

DNA purified with the Maxwell® RSC Plant DNA Kit is ready for use in downstream applications, including qPCR. PCR using DNA purified with the Maxwell® RSC Plant DNA Kit shows no significant evidence of inhibitors.

In this experiment, tenfold dilutions of DNA purified from 20mg of various plant leaf tissues were amplified using qPCR. The ΔCq is calculated as the shift in Cq between each tenfold dilution. The expected ΔCq value for a tenfold dilution is around 3.3. The observed ΔCq value for the Maxwell® RSC Plant DNA samples indicates minimal inhibition.


13433MA-W

High DNA Yield from Various Plant Samples

The cellulose-based particles in the Maxwell® Kits have higher binding capacity than silica-based DNA purification methods, giving consistently high yields. In the samples shown here, DNA was isolated from 20mg of plant tissues (Arabidopsis, Balsam, Corn and Soybean). The eluted DNA was quantified using the QuantiFluor® dsDNA Dye and the Quantus™ Fluorometer.

13432MA-W

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Tail Lysis Buffer (TLA)

 A509C 1 × 25ml View Product

RNase A Solution

 A797C 1 × 1ml 4mg/ml View Product

Elution Buffer

 A828D 1 × 20ml View Product

Maxwell® RSC Cartridge (RSCB)

 AS140A 48 × 1 each

Elution Tubes (0.5ml)

 AS620A 1 × 50/pk View Product

Nuclease-Free Water

 P119C 1 × 25ml View Product

RSC Plungers

 AS1670 1 × 48/pack View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.

Resources

Software and Firmware

Posters

Similar Products

Maxwell® RSC Plant RNA Kit

Extracts RNA from plant tissue samples using the Maxwell® RSC Instrument.

AS1500

Maxwell® RSC PureFood GMO and Authentication Kit

Safe, automated DNA purification from food and feed samples.

AS1600

Frequently Used With

Maxwell® RSC Instrument

Benchtop instrument for automated nucleic acid purification with integrated quantitation. For Research Use Only.

AS4500

Quantus™ Fluorometer and NGS Starter Package

Two fluorescence channels for nucleic acid and protein quantitation. Available in bundle for NGS applications.

E6150, E5150

QuantiFluor® dsDNA System

Specific, sensitive dsDNA quantitation system.

E2670, E2671

Maxwell® RSC 48 Instrument

Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.

AS8500

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