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Viral RNA/DNA Concentration and Extraction Kits for Wastewater

a2991-vacuum-equipment-3
as1831-maxwell-rsc-enviro-tna-kit-3
a2981bindingbuffer1320ml3
mc1501bindingbuffer230ml3
a1442proteasesolution30ml3
A8281_Elution-Buffer--50ml
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Direct Capture and Purification of Viral DNA/RNA From Wastewater

  • Process 40ml input sample volume of wastewater
  • Extract TNA in 2 hours or less and efficiently remove PCR inhibitors
  • Choose from three formats to fit your sample throughput: Manual (A2991); Maxwell® automation (AS1831); or high-throughput, plate-based automation (AX9190)
  • For new testers we offer a startup bundle including the vacuum manifold hardware (110V or 220V) for concentration plus a purification kit (see specs below)
  • For additional voltage options, contact Technical Service

Choose a Product

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Catalog number selected: A2991

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Viral RNA/DNA Concentration and Extraction Kits for Wastewater
Wizard® Enviro TNA Kit/25 preps
Your price:
Change Configuration

Direct Capture of SARS-CoV-2 RNA in 2 Hours

The Enviro Total Nucleic Acid Kits for Wastewater include reagents and consumables to concentrate and purify total nucleic acids (TNA) in one convenient kit. Our unique vacuum-based direct capture method simplifies sample processing by concentrating and capturing viral TNA quicker than precipitation methods without the use of cumbersome ultracentrifugation, while achieving high and consistent yield. The entire process from sample to purified TNA takes less than 2 hours and is scalable and adaptable to your needs. The process also removes most PCR inhibitors, so the resulting TNA can be used directly for amplification of SARS-CoV-2 RNA using the SARS-CoV-2 RT-qPCR Kit for Wastewater.

We offer three kit formats based on sample throughput. Direct capture of nucleic acids is followed by purification using mini spin columns or benchtop automation using Maxwell® Instruments. A plate-based high-throughput kit is also available. An optional step can be included to extract nucleic acids from solid samples.

Choose A Format For Your Sample Throughput

Manual: Vacuum + Spin Column

The Wizard® Enviro Total Nucleic Acid Kit (A2991) allows you to get purified TNA in 90 minutes or less with a simple protocol. First, the TNA is directly captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is further cleaned using PureYield™ Minicolumns. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA. 

Maxwell® Automation: Vacuum + Maxwell® Extraction

The Maxwell® RSC Enviro Total Nucleic Acid Kit (AS1831) allows you to get purified TNA in 2 hours with vacuum-based direct capture of TNA followed by automated TNA extraction using Maxwell® Instruments. The Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples in a single run. Viral TNA is first captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is loaded into the Maxwell® RSC Instrument for walkaway RNA extraction. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA. 

High-Throughput Automation: Plate-Based

The Maxwell® HT Environmental TNA Kit (AX9190) can be used for high-throughput, automated extraction of viral TNA from wastewater. After an initial concentration with another method, samples are extracted in 96-well plates using magnetic separation. The kit can be used with any automated high-throughput platform. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.

High-Throughput Concentration and Extraction: Plate-Based

The Maxwell® HT Enviro Concentration and TNA Extraction Kit (CS344301) provides magnetic particle-based concentration of virus with Ceres Nanotrap® particles from 10ml of wastewater followed by extraction of TNA from the concentrate. The reagents are formatted for use with laboratory automation for walkaway sample processing with minimal user intervention. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.

Simple Direct Capture Protocol

The filter-based Direct Capture method allows rapid purification and concentration of viral nucleic acids from large volumes of wastewater samples.

Overview of direct capture method for rapid purification and concentration of viral nucleic acid from wastewater

Direct Capture Results in Higher Yield Than PEG/NaCl

wastewater concentration SARS-CoV-2 RNA yield comparison

Preliminary data shows the direct capture method resulted in 30 times higher SARS-CoV-2 RNA yield compared to PEG/NaCl. Wastewater samples were collected from a major US city.

Select Which Product is Right for You

Learn more about wastewater surveillance methods and tools.

Cat.# Name Concentration Method Input Sample Volume Intended Throughput Size Format
A2991 Wizard® Enviro TNA Kit Vacuum-Based 40ml <16 25 preps Manual Spin Column
AS1831 Maxwell® RSC Enviro TNA Kit Vacuum-Based 40ml 1–48 48 preps Semi-Automated
CS347901 Maxwell® RSC Enviro Nanotrap® A TNA Kit Ceres Nanotrap® Microbiome A particles 10ml >48 48 preps Semi-Automated
CS344301 Maxwell® HT Enviro Nanotrap® A TNA Kit Ceres Nanotrap® Microbiome A particles 10ml >48 4 × 96 preps High-Throughput
AX9190 Maxwell® HT Enviro Kit Not Included 1ml >48 4 × 96 preps High-Throughput

What Customers Are Saying

“We are very happy to have implemented the large volume extraction kits in our analysis of wastewater for SARS-CoV-2. The integration of concentration and extraction steps has been instrumental in expediting our workflow. The kits also provide higher reproducibility between biological replicates compared to the method we were using previously.”

Jeseth Delgado Vela
Asst. Professor, Howard University

“We have tested the new Promega Wastewater Kit and were very pleased with results. In my side-by side comparison, the Promega Wastewater Kit has demonstrated way higher viral RNA recovery. We are planning to use it for all future wastewater work.”

Sergey Balashov
PCR Laboratory Director, EMSL Analytical

Have questions? We're here to help!
 

Contact Us

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: A2991 Change Configuration

What's in the box?

Item Part # Size Available Separately

Binding Buffer 1 (BBD)

 A2981 1 × 320ml View Product

Binding Buffer 2 (BBE)

 MC1501 1 × 30ml View Product

Protease Solution

 A144C 1 × 13ml View Product

Column Wash 1 (CWE)

 A296A 1 × 85.3ml

Column Wash 2 (RWA)

 A297A 1 × 206ml

Nuclease-Free Water

 P1195 1 × 150ml View Product

PureYield™ Binding Columns

 A245A 5 × 5 each

PureYield™ Minicolumns

 A247C 1 × 25/pk

PureYield™ Collection Tubes

 A167C 1 × 25/pk

Elution Tubes

 Z200C 1 × 50/pk

Reservoir Extension Funnel

 A3041 1 × 25/pk View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: AS1831 Change Configuration

What's in the box?

Item Part # Size Available Separately

Column Wash 1 (CWE)

 A296A 2 × 85.3ml

Column Wash 2 (RWA)

 A297A 2 × 206ml

Nuclease-Free Water

 P1195 1 × 150ml View Product

PureYield™ Binding Columns

 A245C 12 × 4 each

Elution Tubes (0.5ml)

 AS620A 1 × 50/pk View Product

Maxwell® RSC Cartridge (RSCJ)

 AS159A 48 × 1 each

RSC Plungers

 AS1670 1 × 48/pack View Product

Elution Tubes

 Z200C 1 × 50/pk

Binding Buffer 1 (BBD)

 A2981 2 × 320ml View Product

Binding Buffer 2 (BBE)

 MC1501 2 × 30ml View Product

Protease Solution

 A1442 1 × 30ml View Product

Reservoir Extension Funnel

 A3041 2 × 25/pk View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: AX9190 Change Configuration

What's in the box?

Item Part # Size Available Separately

Protease Solution, Custom

 AX918A 1 × 60ml

Cell Lysis Buffer (CLD)

 A173C 1 × 160ml View Product

Resin

 A175B 4 × 5.5ml View Product

Wash Buffer (WBA)

 A176C 3 × 350ml View Product

CTAB Buffer

 MC1411 1 × 100ml View Product

25mM Tris-HCl (pH 8.0)

 A2641 2 × 60ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

DD

Specifications

You are viewing: A3060 Change Configuration

What's in the box?

Item Part # Size Available Separately

Wizard® Enviro TNA Kit

 A2991 1 × 25 preps

Welch Vacuum Pump for Continental Europe Electrical

 A6722 1 × 1 each

Eluator™ Vacuum Elution Device

 A1071 1 × 4 each View Product

Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

 A7231 1 × 1 each View Product

Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper

 A3110 1 × 1 each View Product

Polypropylene Vacuum Flask, 1,700ml

 AS1839 1 × 1 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Specifications

You are viewing: A3080 Change Configuration

What's in the box?

Item Part # Size Available Separately

Maxwell® RSC Enviro TNA Kit

 AS1831 1 × 48 preps

Welch Vacuum Pump for Continental Europe Electrical

 A6722 1 × 1 each

Eluator™ Vacuum Elution Device

 A1071 1 × 4 each View Product

Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

 A7231 1 × 1 each View Product

Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper

 A3110 1 × 1 each View Product

Polypropylene Vacuum Flask, 1,700ml

 AS1839 1 × 1 each

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Software and Firmware

Other Resources

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Frequently Used With

Maxwell® RSC Instrument

Benchtop instrument for automated nucleic acid purification with integrated quantitation. For Research Use Only.

AS4500

Maxwell® RSC 48 Instrument

Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.

AS8500

Vac-Man® Jr. Laboratory Vacuum Manifold

For rapid, effective nucleic acid purification. Process two samples at a time.

A7660, A3110

Eluator™ Vacuum Elution Device

Eliminates need for centrifugation when eluting DNA with PureYield™ Systems.

A1071

Vac-Man® Laboratory Vacuum Manifold

For fast, effective nucleic acid purification. Process up to 20 samples simultaneously.

A7231

Binding Buffer 1 (BBD)

Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.

A2981

Binding Buffer 2 (BBE)

Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.

MC1501

Protease Solution

Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.

A1442

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