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Lumit® TNF-α (Human) Immunoassay

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No-Wash Immunoassay for TNF-α Detection

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a plate-reading luminometer
  • Scalable to 96- or 384-well plates

Size

Catalog number selected: W6050

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Lumit® TNF-α (Human) Immunoassay
100 assays
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Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® TNF-α Immunoassay quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® TNF-α Immunoassay Works

Primary antibodies to TNF-α were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of TNF-α, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to TNF-α levels.

How the Lumit™ IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ IL-2 Immunoassay Workflow

Large Dynamic Range of Lumit® TNF-α Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

lumit tnf-alpha standard curve 17850ma

Assay

TNF-α Human Immunoassay

Limit of Detection (LOD)

2.9pg/ml (3 SD above background)

Dynamic Range

18.2pg/ml – 25ng/ml

Minimal Detectable Dose (MDD)

2.0pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Sensitive Detection of TNF-α Without Sample Dilution

Data showing sensitive detection of TNF-α without sample dilution

When treated with a Cell Stimulation Cocktail (CSC), human PBMCs release TNF-α. After treatment for 24, 48 or 72 hours, Lumit® TNF-α Human Immunoassay reagents were added directly to cell wells to measure TNF-α release. No sample dilution was required to measure quantities of TNF-α release.

Multiple Cytokine Detection by Split-Sample Analysis

Split-sample analysis for detection of IL-2, IL-6, IFN-γ and TNF-α using corresponding Lumit™ Cytokine Immunoassay.18001ma

Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit® Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.

Featured Application Note

CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells

Explore a multiplexing approach that unlocks the full potential of CAR T cell therapy with our Lumit® IFN-γ and TNF-α (Human) Immunoassays.

View Application Note

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Specifications

You are viewing: W6050 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Human TNF-α Standard

 W137A 1 × 25μl 10μg/ml

Anti-hTNF-α mAb-SmBiT, 1000X

 W138A 1 × 15μl

Anti-hTNF-α mAb-LgBiT, 1000X

 W139A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6052 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Human TNF-α Standard

 W137A 1 × 25μl 10μg/ml

Anti-hTNF-α mAb-SmBiT, 1000X

 W138A 5 × 15μl

Anti-hTNF-α mAb-LgBiT, 1000X

 W139A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6051 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Human TNF-α Standard

 W137A 1 × 25μl 10μg/ml

Anti-hTNF-α mAb-SmBiT, 1000X

 W138B 1 × 150μl

Anti-hTNF-α mAb-LgBiT, 1000X

 W139B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

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