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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Lumit® IL-6 (Human) Immunoassay

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No-Wash Immunoassay for IL-6 Detection

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a plate-reading luminometer
  • Scalable to 96- or 384-well plates

Size

Catalog number selected: W6030

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Lumit® IL-6 (Human) Immunoassay
100 assays
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Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® IL-6 Immunoassay quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® IL-6 Immunoassay Works

Primary antibodies to IL-6 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-6, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-6 levels.

How the Lumit™ IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ IL-2 Immunoassay Workflow

Large Dynamic Range of Lumit® IL-6 Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

lumit il-6 standard curve 17847ma

Assay

IL-6 Human Immunoassay

Limit of Detection (LOD)

7.5pg/ml (3 SD above background)

Dynamic Range

18.2pg/ml – 25ng/ml

Minimal Detectable Dose (MDD)

5.0pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Rapid Detection of IL-6 Release from PBMCs

Comparison of IL-6 release from PBMCs using Lumit IL-6 Human Immunoassay and standard ELISA.17953ma-w

The Lumit® IL-6 (Human) Immunoassay (left) and standard ELISA (right) were used to quantify IL-6 release from PBMCs treated with three inducers (cell stimulation cocktail, lipopolysaccharide or R848) for up to 72 hours. The results are similar, while the Lumit® Immunoassay was completed in one fifth the time of the ELISA, with no transfer and wash steps or plate washer needed.

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Specifications

You are viewing: W6030 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Human IL-6 Standard

 W128A 1 × 25μl 10μg/ml

Anti-hIL-6 mAb-SmBiT, 1000X

 W129A 1 × 15μl

Anti-hIL-6 mAb-LgBiT, 1000X

 W130A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6032 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Human IL-6 Standard

 W128A 1 × 25μl 10μg/ml

Anti-hIL-6 mAb-SmBiT, 1000X

 W129A 5 × 15μl

Anti-hIL-6 mAb-LgBiT, 1000X

 W130A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6031 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Human IL-6 Standard

 W128A 1 × 25μl 10μg/ml

Anti-hIL-6 mAb-SmBiT, 1000X

 W129B 1 × 150μl

Anti-hIL-6 mAb-LgBiT, 1000X

 W130B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

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GloMax® Discover System

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