qPCR Kits: GoTaq® qPCR and RT-qPCR Systems

Reverse Transcription and qPCR in One Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Other features provided by the 1-Step System include:

• Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
• Exceptional room-temperature setup, allowing easy automation and high-throughput detection.

Two-Step Reverse Transcription and qPCR

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.