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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

BHB-Glo™ (Ketone Body) Assay

je9500-bhb-glo-ketone-body-assay-5-ml
je9600-bhb-glo-ketone-body-assay-50-ml
je9500-bhb-glo-ketone-body-assay-5-ml
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Easy, Sensitive Beta-Hydroxybutyrate Assay Kit

  • Compatible with cells, media, tissue and serum samples
  • In-well sample preparation protocol streamlines workflow
  • Scalable to 384-well plates for HTS applications

Size

Catalog number selected: JE9500

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BHB-Glo™ (Ketone Body) Assay
5ml
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Efficient Detection of BHB in Diverse Samples

The BHB-Glo™ (Ketone Body) Assay is a fast and sensitive method for detecting β-hydroxybutyrate (BHB) from various biological samples, such as cultured cells, media, tissue homogenates and serum. Based on bioluminescent technology, the BHB-Glo™ Assay can detect subtle changes in ketosis as well as ketone body consumption and secretion. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation, spin columns and deproteinization. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.

How the BHB-Glo™ Assay Works

Diagram illustrating how the BHB-Glo™ Assay works.

β-Hydroxybutyrate (BHB) Dehydrogenase catalyzes the oxidation of BHB, simultaneously reducing NAD+ to NADH. In the presence of NADH, Reductase converts the proluciferin Reductase Substrate into luciferin. This reaction is detected by Ultra-Glo™ Recombinant Luciferase, which emits light proportionate to the amount of BHB in the sample.

Simple Add-and-Read Protocol Saves Time

Diagram illustrating the BHB-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in BHB

Graph showing the linear relationship between luminescence and BHB concentration.



Sensitivity (S/B >5)

800nM

Limit of Detection (S/N >3)

50nM

Linear Range

50nM to 25μM

Maximum Assay Window (S/B)

>200

Measure BHB Secretion from Hepatocytes




The BHB-Glo™ Assay can be used to monitor intracellular and extracellular BHB levels in cell culture. In the data shown, HepG2 cells were plated in a 96-well plate, and then media samples were removed at prescribed time points to monitor BHB secretion over time. Extracellular BHB was measured using the BHB-Glo™ Assay. Greater than sixfold signal above background was observed at 24 hours.

Bar chart showing luminescence measured at 0, 6 and 24 hours for measuring extracellular BHB concentration from HepG2 cell culture with the BHB-Glo™ Assay.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

Explore the Cellular Energy Metabolism Portfolio

Protocols

Complete Protocol

Specifications

You are viewing: JE9500 Change Configuration

What's in the box?

Item Part # Size Concentration

β-Hydroxybutyrate Dehydrogenase

 J414A 1 × 100μl

β-Hydroxybutyrate, 10mM

 J415A 1 × 50μl

Reductase

 G884A 1 × 55μl 6mg/ml

Reductase Substrate

 G885A 1 × 55μl

Luciferin Detection Solution

 J135A 1 × 5ml

NAD

 J136B 1 × 275μl

Neutralization Buffer

 J153A 1 × 15ml

NADP+

 J154B 1 × 100μl

0.6N HCl

 J402A 1 × 15ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: JE9600 Change Configuration

What's in the box?

Item Part # Size Concentration

β-Hydroxybutyrate Dehydrogenase

 J414B 1 × 1ml

β-Hydroxybutyrate, 10mM

 J415A 1 × 50μl

Reductase

 G884B 1 × 275μl 6mg/ml

Reductase Substrate

 G885B 1 × 275μl

Luciferin Detection Solution

 J135B 1 × 50ml

NAD

 J136C 1 × 1ml

Neutralization Buffer

 J153A 1 × 15ml

NADP+

 J154C 1 × 500μl

0.6N HCl

 J402A 1 × 15ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Articles

Posters

Other Resources

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Frequently Used With

RealTime-Glo™ MT Cell Viability Assay

A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours.

G9711, G9712, G9713

CellTiter-Fluor™ Cell Viability Assay

A non-lytic, fluorescent cell viability assay with multiplex capability.

G6080, G6081, G6082

CellTiter-Glo® 2.0 Cell Viability Assay

Updated CellTiter-Glo® Cell Viability Assay with improved reagent stability. Quantifies cell proliferation based on ATP detection.

G9241, G9242, G9243, MG1010

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

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