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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Glycerol-Glo™ Assay

J3150_Glycerol-Glo--Assay_3
J3151_Glycerol-Glo--Assay_3
J3150_Glycerol-Glo--Assay_3
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Simple, Sensitive Glycerol Detection Assay for a Variety of Biological Samples

  • Works with lysed cells, tissues, medium and serum samples
  • Perform directly in-well
  • Linear glycerol detection up to 80µM

Size

Catalog number selected: J3150

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Glycerol-Glo™ Assay
5ml
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Rapid and Sensitive Glycerol Detection in Biological Samples

The Glycerol-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of glycerol in a variety of biological samples, including cells grown in monolayer or 3D structures, cell culture medium, tissues and serum samples. Glycerol is often measured as the product of lipolysis, where it is released from triglycerides. Glycerol is also a substrate or product of many other enzymatic or metabolic processes that can be studied with the glycerol assay. Any processes that result in changes in glycerol concentration, both extracellular and intracellular, can be studied with the Glycerol-Glo™ Assay.

How the Assay Works

The Glycerol-Glo™ Assay measures glycerol in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. Glycerol kinase and glycerol-3-phosphate dehydrogenase are used to generate NADH.

In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP and the amount of light produced is proportional to the amount of glycerol in the sample.

Diagram showing how the glycerol-glo assay detects glycerol

Simple, Flexible Sample Preparation

Glycerol Release from Adipocytes

The Glycerol-Glo™ Assay can be used with cultured cells, including 3D cell culture models, homogenized tissue samples, cell culture medium samples and serum or plasma samples.

No organic extraction is required. The Glycerol Lysis Reagent effectively extracts glycerol from your sample in a single reagent addition without extreme heat, centrifugation steps or multi-step organic extraction protocols.

Glycerol release from adipocytes detected using the Glycerol-glo assay.
Adipocytes were differentiated from 3T3L1-MBX fibroblasts. Medium was removed and the cells were washed twice with PBS. Medium containing 2% fatty acid-free BSA and 5μM triacsin C to promote extracellular glucose accumulation was added, with or without 10μM isoproterenol to induce stimulated or control conditions, respectively. At the indicated time points, aliquots of medium were removed and assayed for glycerol. The glycerol detection assay is used to quantitate lipolysis in adipocytes.

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: J3150 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase Substrate

 G885A 1 × 55μl

Glycerol Lysis Solution

 J315A 1 × 10ml

Kinetic Enhancer

 J316A 1 × 50μl

Glycerol Detection Solution

 J317A 1 × 5ml

Glycerol Standard

 J324A 1 × 500μl 20mM

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: J3151 Change Configuration

What's in the box?

Item Part # Size Concentration

Reductase Substrate

 G885B 2 × 275μl

Glycerol Lysis Solution

 J315B 1 × 100ml

Kinetic Enhancer

 J316B 1 × 500μl

Glycerol Detection Solution

 J317B 1 × 50ml

Glycerol Standard

 J324A 1 × 500μl 20mM

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Articles

Posters

Other Resources

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