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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

RealTime-Glo™ Extracellular ATP Assay

How To Measure Immunogenic Cell Death In Real Time
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Live-Cell Kinetic Assay for Measuring Extracellular ATP

  • Get more data per well with real-time monitoring
  • Just add, mix, measure; no supernatant sampling
  • Ideal for high-throughput applications

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Catalog number selected: GA5010

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RealTime-Glo™ Extracellular ATP Assay
200 assays
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Monitor Immunogenic Cell Death in Real Time

The RealTime-Glo™ Extracellular ATP Assay is a bioluminescent assay designed for kinetic monitoring of ATP released from dying, stressed or activated cells. Extracellular ATP can function as a damage associated molecular pattern (DAMP) and is a key biomarker for determining whether a treatment induces immunogenic cell death, a specialized form of cell death that results in an immune response.

The RealTime-Glo™ Extracellular ATP Assay allows you to continually monitor extracellular ATP so you won’t miss crucial time points. It is compatible for use in 384-well plates, making it ideal for high-throughput screening applications.

Simple Protocol for Measuring Extracellular ATP

Set up one plate to collect all the time points you need! First, reconstitute the reagent with cell culture medium and add to cells at the time of treatment. Then, read the luminescent signal continuously for 24 hours or longer using a standard plate-reading luminometer. No supernatant sampling required.

RealTime-Glo Extracellular ATP Assay Workflow

Kinetic Monitoring of Immunogenic Cell Death

Kinetic Monitoring of Immunogenic Cell Death

U937 cells were treated with a dilution of mitoxantrone, an anthracycline shown to induce immunogenic cell death (ICD). RealTime-Glo™ Extracellular ATP Assay Reagent was added, and the assay plate was placed in a plate reader set at 37°C. Luminescence was collected every 10 minutes for 24 hours.

Compatible with 2D and 3D Cell Models 

Compatible with 2D Cell Models
Compatible with 3D Cell Models

HCT-116 cells were grown in monolayer (left) and in spheroids using ultra-low attachment plates (right). Cells were treated with dilutions of staurosporine, and the RealTime-Glo™ Extracellular ATP Assay Reagent was added. The timing for ATP release differs between monolayer and spheroid cell culture, and the reagent is compatible with both cell culture models.

Want to measure ATP as a marker of cell viability?

Learn about the CellTiter-Glo® 2.0 Assay, a stable, single-reagent ATP assay.

Also Available: Lumit® HMGB1 Human/Mouse Immunoassay

The Lumit® HMGB1 Immunoassay detects HMGB1 directly in cell culture samples in 90 minutes or less and streamlines your DAMP and immunogenic cell death research. Use this assay to complement the RealTime-Glo™ Extracellular ATP Assay for a more complete picture of immunogenic cell death.

Protocols

Complete Protocol

Specifications

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What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501A 1 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: GA5011 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501B 1 × 1 vial

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: GA5012 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

 GA501A 10 × 1 vial

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Articles

Other Resources

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