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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Lumit® IL-1β Human/Mouse Immunoassay

W6010_Lumit-Human-IL-1beta-Immunoassay_3
W6011_Lumit-Human-IL-1beta-Immunoassay_3
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No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates

Choose an antibody

Size

Catalog number selected: W6010

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Lumit® IL-1β Human/Mouse Immunoassay
Human/100 assays
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Quantify Inflammasome Activation Without Tedious Wash Steps

The Lumit® IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® IL-1β Immunoassay Works

Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.
16522ma

Simple One-Plate Protocol, No Wash Steps!

16545ma

Large Dynamic Range of Lumit® Detection

The picomolar to nanomolar linear range of Lumit® detection covers expected concentrations for most cell models.

Linear range of lumit IL-1b human immunoassay
Linear range of lumit IL-1b mouse immunoassay

Assay

IL-1β (Human) Immunoassay

IL-1β (Mouse) Immunoassay

Limit of Detection (LOD)

10pg/ml (3 SD above background)

8pg/ml (3 SD above background)

Dynamic Range

22pg/ml – 40ng/ml

11pg/ml – 20ng/ml

Minimal Detectable Dose (MDD)

7pg/ml (2 SD above background)

5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

High-Throughput NLRP3 Inflammasome Inhibitor Screening

The add-and-read format of the Lumit® IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
IL-1b inhibitor high throughput screening

The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit® IL-1β (Human) Immunoassay was performed after 5 hours.

Featured Publication

This peer-reviewed study "Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays" examines the direct engagement and functionality of NLRP3 pathway activity in cells using several mechanistic assays, including the Lumit® IL-1β Immunoassay and Caspase-Glo 1 Inflammasome Assay.

nlrp3-martha-obrien-paper

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Specifications

You are viewing: W6010 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117A 1 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W6011 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117B 1 × 150μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W6012 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Human IL-1β Standard

 W116A 1 × 25μl 10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

 W117A 5 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

 W118A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7010 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 1 × 160μl

Lumit® Detection Buffer B

 VB406A 1 × 3.2ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120A 1 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121A 1 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7011 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405B 1 × 1.25ml

Lumit® Detection Buffer B

 VB406B 1 × 25ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120B 1 × 150μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121B 1 × 150μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Specifications

You are viewing: W7012 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit® Detection Substrate B

 VB405A 5 × 160μl

Lumit® Detection Buffer B

 VB406A 5 × 3.2ml

Mouse IL-1β Standard

 W119A 1 × 25μl 10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

 W120A 5 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

 W121A 5 × 15μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

Resources

Articles

Posters

Other Resources

Similar Products

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Lumit® IL-6 (Human) Immunoassay

Quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol.

W6030, W6032, W6031

Lumit® Active IL-18 (Human) Immunoassay

Quantitatively measure active IL-18 in cell culture samples using a simple, no-wash protocol completed in 70 minutes or less.

Caspase-Glo® 1 Inflammasome Assay

A homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.

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Frequently Used With

LDH-Glo™ Cytotoxicity Assay

Bioluminescence-based LDH assay for sensitive detection of cytotoxicity in samples low in cell number, including 3D microtissues.

J2380, J2381

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

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