The Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect a wide range of proteins, phosphoproteins or small molecules of interest, provided the appropriate primary antibodies (supplied by the user) are available. We recently developed a new lysis buffer (Lumit® Lysis Buffer II), which broadens the range of cellular targets (see table below) that can be interrogated using the Lumit® Immunoassay Cellular System.
Also available are Complete Assays, which include sets of antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK as well as several others. These are available as early access materials.
To save you time, we have evaluated a number of common targets using commercially available primary antibodies. We have expanded the number of Application Notes to include targets that are now accessible due to the new lysis buffer. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.