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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Trypsin/Lys-C Mix, Mass Spec Grade

V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
Protein Cleavage with Trypsin-Lys-C
V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
customize-this-small

Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

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Trypsin/Lys-C Mix, Mass Spec Grade
20μg
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Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Trypsin digest illustration.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protocols

Complete Protocol

Specifications

You are viewing: V5071 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 1 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5072 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507B 1 × 100μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5073 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 5 × 20μg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Posters

Other Resources

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Streamlined protocol for fast, efficient protein digestion.

VA1060, VA1061

rAsp-N, Mass Spec Grade

Cleavage site: N-terminal of Asp. Optimal pH: 6–9.

VA1160

Frequently Used With

Arg-C, Sequencing Grade

Cleavage site: C-terminal of Arg. Optimal pH: 7.6–7.9.

V1881

Asp-N, Sequencing Grade

Cleavage site: N-terminal of Asp. Optimal pH: 4–9.

V1621

Chymotrypsin, Sequencing Grade

Cleavage sites: C-terminal of Tyr, Phe, Trp. Optimal pH: 7–9.

V1061, V1062

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