GloMax® Galaxy Bioluminescence Imager

A Bioluminescence Microscope that Enables Functional Imaging of NanoLuc® Technologies

Research Use Only Device.
Luminescence, fluorescence and brightfield imaging capabilities
Image NanoLuc® Luciferase technologies (NanoBiT, HiBiT, NanoBRET) in living and fixed cells and tissues
Study protein dynamics and cellular physiology in real time

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Imager including PC, Monitor, Mouse and Keyboard Imager Only

Catalog Number: GM4000

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Catalog Number: GM4005

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Visualize Your NanoLuc® Luciferase Assays with Bioluminescence Imaging

GloMax® Galaxy Bioluminescence Imager is a fully equipped microscope designed to visualize NanoLuc® Luciferase chemistries. Transform your microplate assay results into beautiful and illuminating images through bioluminescence imaging.

Developed for the visualization of all NanoLuc® technologies, including HiBiT, NanoBiT and NanoBRET.
Ideal for assay development; validate the same bioluminescent assay reporter used in your system or workflow.

NanoLuc® Luciferase technologies enable a variety of protein reporter applications. GloMax® Galaxy enables the visualization of:

Protein:protein interactions
Protein localization and translocation
Protein degradation and stability
Ligand:protein interactions (target engagement)
Targeted cell killing

GloMax® Galaxy luminescence image of EGFR protein fusion with Nano-Glo® HiBiT.

Image Low-Abundance Endogenous Proteins

Bioluminescence enables the imaging of low-abundance endogenous proteins. Due to the lower photon flux generated through bioluminescence, very few photons are required to observe a reporter tag. Though the low photon flux requires significantly longer exposure times depending on expression level when compared to fluorescence imaging, there is minimal background noise with bioluminescence imaging due to the lack of autofluorescence and autoluminescence in samples.

Relative Luminescence Units (RLU) are commonly used on a plate reader to indicate lower expressing proteins. Due to the signal-to-noise background of bioluminescence, one can observe very low expression targets by simply exposing the sample for longer periods of time.

Imaging low-abundance proteins through bioluminescence. Panel A. Low-abundance endogenous proteins show lower luminescent signals. Relative luminescent units (RLUs) from NanoBiT®-fusion proteins show a 2 log range in luminescent signal between high-abundance (CFL) and low-abundance (HDAC6) endogenous proteins. Panel B. Images of low- and high-abundance endogenous NanoBiT®-fusion proteins captured on the GloMax® Galaxy. HiBiT was inserted into the genomic locus of the target proteins via CRISPR/CAS9 in HeLa cells. LgBiT was expressed ectopically, and binary complementation yields the target protein-NanoBiT® fusion. Image of the high-abundance CFL required a 1-minute exposure. Image of the low-abundance HDAC6 required 3-minute exposure.

Panel A. Bar graph showing RLU comparison of high- versus low-abundance NanoBiT®-fusion proteins. Panel B. Images of low- and high-abundance endogenous NanoBiT®-fusion proteins captured on the GloMax® Galaxy.

Monitor Protein Kinetics Over Time

One of the major benefits of bioluminescent imaging is the inherent stability and sustainability of the bioluminescent signal, which unlike fluorescent tags, does not require external excitation. This lack of external excitation reduces the risk of phototoxicity and photobleaching, common issues that can adversely affect cell viability and signal integrity over time.

Bioluminescent tags allow repeated imaging sessions over days, weeks or months without altering the physiological state of the system under study. Also, subtle changes in bioluminescent signal reflective of protein changes are more easily observed due to the lower photon flux of bioluminescence. This is optimal for studying targeted protein degradation.

Targeted protein degradation over time. HEK293 cells expressing endogenous HiBiT-tagged GSPT1 and stably expressing LgBiT were treated with CC-885 degrader or DMSO control treatment. Assayed with Nano-Glo® Vivazine™ Live Cell Substrate and imaged over 5 hours using GloMax® Galaxy with the Stagetop Incubator/Controller, GloMax® Galaxy.

Images showing targeted protein degradation over time.

Service and Support

One Call Supports It All

Promega supplies both the reagents and the instrument, so one call to Promega answers any questions you may have about assay chemistries or instrument performance.

Contact Technical Services

Ensure Minimal Instrument Downtime

Field Support and Loaner Programs
Service Packages
IQ and OQ Packages

Warranty and Service Agreements

Backed by a one-year warranty.
Additional warranty and service agreements are available.
For more information, contact Promega Technical Services.

Instrument Service and Support Instrument Registration

Protocols

Complete Protocol

GloMax® Galaxy Bioluminescence Imager Operating ManualPDF (8203 KB) – English

Design Features and Specifications

GloMax® Galaxy Specifications

Capture Modes	Luminescence, BRET, Fluorescence and Brightfield
Excitation Source	LED, transillumination
Dimensions (W × H × D)	14.7in × 18.8in × 21.0in 37.3cm × 47.7cm × 53.3cm
Weight	62lb (28kg)
Resolution Limit	1.3–2.0µm
Power Requirements	100–240V AC, 50/60Hz
Digital Zoom	Up to 100X
Objective	Nikon 20X Plan APO Lambda D, 0.75 NA, 1mm WD
System Magnification	10.3X
Sensor and Pixel Size	CMOS, 7 megapixel, cooled to –25°C, low noise, >70% quantum efficiency, 4.5µm × 4.5µm pixel size, up to 60-minute exposure
Pixel Size	3200 × 2200 pixels, 4.5µm × 4.5µm pixel size
Sample Vessels	Slide, microchamber, 35mm dish, 6-, 12-, 24- and 96-well plates
Maximum Field of View	1.4mm × 0.95mm
Focus Mechanism	Motorized, with manual focus to submicron resolution (0.3125µm)
Environment Control	Optional: Stagetop chamber and controller with built-in gas mixer
Use Restrictions	Research Use Only Device.

Stagetop Incubator/Controller, GloMax® Galaxy

Manufacturer	Tokai Hit
Dimensions	151mm × 263mm × 196mm
Weight	4.1kg
Controller	Provides electronic control of temperature and gas
PC Software	Data logging
Stagetop Chamber	Top heater equipped with glass heater to prevent condensation, external sensor, and vessel holders for well plates, 35mm and 50/60mm dishes, chamber slides, chambered cover glass
Sample Temperature Range	30°C to 40°C in 0.1°C increments; accuracy within +/– 0.1°C
Humidity	Up to 85% Rh; integrated water reservoir
CO₂ Concentration Range	5.0%–20.0%; accuracy within +/– 0.1°C
Input Gas Pressure	Using a 100% CO₂ cylinder: 0.1MPa–0.15MPa
Output Gas Pressure	160ml/minute
Power for Controller	100–240V AC, 50/60Hz; maximum consumption 100W
Not Included. Must be provided by user.	100% CO₂ cylinder Dual valve regulator 4.0mm ID Picso tubing or 6.0mm ID silicon tubing

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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