Spectrum Compact CE System
An Affordable Benchtop Instrument for Sanger Sequencing and Fragment Analysis
- 8-dye-compatible CE analysis
- Single-base resolution
- Integrated touch screen operation
- Plug-and-Play prefilled consumables
Catalog Number:
Choose a System
Catalog Number: CE1304
Catalog Number: CE1307
Catalog Number: CE1305
Benchtop, Personal CE System
The Spectrum Compact CE System is an integrated and efficient instrument that brings you the independence to perform Sanger sequencing and fragment analysis in your laboratory, under your control, and at your convenience.
It is designed for use with existing sequencing chemistries using fluorescently labeled dideoxynucleotide triphosphates and 4-, 5- and 6-dye commercially available STR kits. It also supports the next generation of 8-color STR kits.
Take Control of Your Experiments
Sequence samples on your own benchtop with single-base-pair resolution and 8-dye fragment analysis multiplex.
Bring Sequencing Capabilities to Your Entire Team
An easy-to-use interface and plug-and-play consumables make sequencing accessible to everyone in your lab.
Sequence Samples Without the Wait
No more waiting to batch samples or depending on a sequencing service.
Get the Support You Want
To get you started, we provide on-site installation and personalized training.
Optimized for a Broad Range of Sequencing and Fragment Analysis Applications
- Human Identification
- Human Cell Line Identification and Authentication
- Cell and Gene Therapy Quality Control
- Mixed Sample Analysis
- Microsatellite Instability (MSI) Analysis
- Multiplex Ligation-Dependent Probe Amplification (MLPA)
- Single-nucleotide Polymorphism (SNP) Genotyping
- Targeted Sanger Sequencing
- Resequencing for Mutation Detection
- NGS Confirmation
- Mitochondrial DNA Sequencing
- BAC Sequencing
- Methylation Sequencing
- Viral Genotyping
- Plasmid Sequencing
- Clone Construction Verification
- Microbial Species Identification
- SARS-CoV-2 Identification
- De novo Sequencing
Want to See Spectrum Compact for Yourself?
Plug-and-Play, Prefilled Consumables
Plug-and-play prefilled consumables with guided software user interface brings capillary electrophoresis capabilities to the hands of any researcher in the laboratory regardless of skill level or expertise.
Not only is the Spectrum Compact CE System simple to set up and operate, but the dedicated consumables are independently packaged, offering significant benefits:
- Manage your own usage lifetimes without any waste. For example, don't limit the lifetime of your capillary array based on buffer or polymer expiration dates or usage levels.
- Visually inspect each consumable (buffer levels and mechanical interactions) to easily troubleshoot issues without replacing an entire consumable set at once.
- Decrease energy usage and avoid potential condensation—Spectrum Compact does not utilize on-board refrigeration or require the instrument to remain powered on for consumable preservation.
A. Capillary Cartridge
- 4-Capillary, 36cm.
- Located behind the oven door.
- Easily installed by snapping into place and lies flat against oven to maintain migration stability.
- Sufficient for 1200 samples (300 injections and no hard-stops).
B. Anode Buffer Cartridge
- Contains the buffer for the anode electrode.
- Sufficient for 320 samples (80 injections).
C. Sample Cartridge
- Holds up to four 8-well strip tubes for a total of 32 samples.
D. Cathode Buffer Cartridge
- Contains the buffer for the cathode electrodes.
- Sufficient for 320 samples (80 injections).
E. Polymer Cartridge
- Contains the respective denaturing polymer (Polymer4 or Polymer7) for Sanger sequencing or fragment analysis.
- Sufficient for 96 samples (24 injections). Only hard-stop is insufficient polymer.
Consumables Information
| Cat. Number | Description | Size |
|---|---|---|
| CE2300 | Spectrum Compact Buffer | 2 pairs |
| CE2301 | Spectrum Compact Cathode Septa Mat | 10 each |
| CE2302 | Spectrum Compact Cathode Retainer | 4 each |
| CE2304 | Spectrum Compact Polymer4; use with software v.6138000-XX* |
4 × 64 wells |
| CE2404 | Spectrum Compact Polymer4; use ONLY with software v.6138200-XX* |
4 × 96 wells |
| CE2307 | Spectrum Compact Polymer7; use with software v.6138000-XX* |
4 × 64 wells |
| CE2407 | Spectrum Compact Polymer7; use ONLY with software v.6138200-XX* |
4 × 96 wells |
| CE2308 | Strip Septa Mat, 8-Well | 24 each |
| CE2332 | Spectrum Compact Strip Base and Retainer, 32-Well | 4 each |
| CE2340 | Spectrum Compact Capillary Cartridge, 4-Capillary 36cm** | 1 each |
| CE2399 | Capillary Preservation Buffer | 10ml |
*Check your software version in "About", located in the footer on the "Main Menu" screen of the Spectrum Compact Control Software. For more information, review the Instrument Information section of the Spectrum Compact CE System Operating Manual #TMD058.
**When used with software v.6138000-XX, Cat.# CE2340, Spectrum Compact Capillary Cartridge, 4-Capillary 36cm, will display 200 injections; when used with software v.6138200-XX, 300 injections will be displayed. There is no hard-stop on capillary cartridge injections.
Small-Footprint Advanced Multiplexing
The Spectrum Compact CE System offers the high performance of a 8-dye-compatible sequencer in a benchtop instrument for fast and affordable results.
These new 8-dye systems enable amplification and detection of more loci from challenging samples, due to the narrower range of product amplicon sizes, resulting in more meaningful analyses.
Electropherogram of 1ng of 2800M Control DNA amplified with the PowerPlex® 35GY System and separated using the Spectrum Compact CE System.
Single-Base Resolution
Spectrum Compact CE System achieves a 1bp resolution, as can be seen in the D12S391 locus in the PowerPlex® ESI 17 Fast System Allelic Ladder. Injection was performed at 1.6kV for 9 seconds.
More Bases in Less Time
Combine ProDye® Terminator Sequencing system with the Spectrum Compact for more bases in less time.
Raw view of entire electropherogram using ProDye® Terminator Sequencing System on the Spectrum Compact CE System.
Accurate and Fast Sequencing
Representative portion of an electropherogram (Panel A) generated using the ProDye® Terminator Sequencing System on a Spectrum Compact CE System. Injection was performed at 1.2kV for 4 seconds with Polymer7; Panel B shows raw data of standard sequencing run.
The Spectrum Compact Control Software provides a simple user interface with a clear display of useful features:
- Easy-to-use operational workflow wizard
- Run set up
- Consumables usage
- Maintenance reminders
- Operations run directly on the instrument touch screen
- Ability to monitor run progress and view analyzed results while a run is in progress
- Pre-loaded assays ready for use
- Export files in .fsa or .ab1 format
Remote Access
The Spectrum Compact Remote Access Software allows a user to connect to a Spectrum Compact CE System from a PC on the same network. You can:
- Easily create/edit/review/delete strip IDs for sample strips.
- Create/edit/review/delete protocols and assays.
- View analysis results and monitor a run in progress, as well as completed runs.
- Download completed runs using a web browser on a PC connected to the instrument, either directly or over a lab network.
To learn more about operating and maintaining the instrument, check out our tutorial videos.
GeneMarker®HID Software for Spectrum CE Systems
GeneMarker®HID Software for Spectrum CE System (GMHID-Spectrum) supports data analysis with all commercially available human identity kits. A range of reporting options are available to meet LIMS and report printing needs, and analysis and display options can be easily configured to support your laboratory's workflow. Other benefits include:
- Database searching and relationship testing modules
- Review tools, such as automated contamination checking and project comparison
- Time savings of at least 25%
- Less analyst intervention
- Supports Spectrum (.promega) and existing CE data (.fsa and .hid files)
The analysis of 8-color chemistry requires the use of GeneMarker®HID for Spectrum Systems.
Linked Navigation: Simply click on a peak or in the table to highlight data details.
Multi-Color View: View/comment/edit all dye channels for an allelic ladder or sample on one screen.
ProView® Sequencing Software
ProView® Sequencing Software is a customizable Sanger sequencing viewer capable of displaying .ab1 files generated on many currently available capillary electrophoresis (CE) sequencers. This software can be used to view and edit sequence information, display electropherograms, generate reports, display embedded information and save edited files in multiple formats.
Service and Support
We understand that your work can’t stop, and we’re dedicated to minimizing any interruptions to your lab operations. On-site installation and operational training are included with purchase of the instrument, and preventive maintenance, applications training, validation and on-site service are also available. Promega scientists are ready to help, and we expect to be there when you need us.
Service
Phone-based and on-site consultation in addition to data analyses and summary reports are the kinds of options for support you can expect.
Installation
As you transition to include the Spectrum Compact CE System in your lab, we will be with you every step of the way. We offer on-site instrument, software and application training as well as timely on-site installation and service.
Maintenance
If you want regular servicing and maintenance, we can take care of you. In addition to standard maintenance contracts, we have additional packages to keep your Spectrum Compact CE System running.
Specifications
Instrument Specifications
| Number of capillaries | 4 |
| Capillary array length | 36cm |
| Number of dye colors | 8 |
| Throughput | Four 8-well strip tubes |
| Computer platform | Integrated touch screen with built-in computer |
| Polymer | Polymer4 and Polymer7 |
| Data File Type | .fsa for fragment analysis and .ab1 for Sanger sequencing |
| Dimensions | 40cm W x 60cm D x 60cm H (15.75in W x 23.62in D x 23.62in H) |
| Weight | 45kg (99lb) |
| Power Input | 100–240VAC, 50/60Hz, 260VA |
Run Module Specifications
| Run Module | Polymer Type | Average Run Time (Minutes) | Typical Contiguous Read Length (Base) |
|---|---|---|---|
| Fast Sequence | Polymer7 | ≤34 | ≥600 |
| Standard Sequence | Polymer7 | ≤68 | ≥800 |
| Run Module | Polymer Type | Average Run Time (Minutes) | Size Range (Base Pairs)* |
|---|---|---|---|
| Fragment Analysis | Polymer7 | ≤35 | 60-600 |
| Fragment Analysis | Polymer4 | ≤44 | 60-600 |
*Size precision 60-600bp <0.16nts
Catalog Number:
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
Spectrum Compact Buffer |
CE2300 | 1 × 2 pairs |
Spectrum Compact Polymer4; use with software v.6138000-XX |
CE2304 | 1 × 4 × 64 wells |
Spectrum Compact Polymer4; use ONLY with software v.6138200-XX |
CE2404 | 1 × 4 × 96 wells |
Spectrum Compact Polymer7; use with software v.6138000-XX |
CE2307 | 1 × 4 × 64 wells |
Spectrum Compact Polymer7; use ONLY with software v.6138200-XX |
CE2407 | 1 × 4 × 96 wells |
Capillary Preservation Buffer |
CE2399 | 1 × 10ml |
Spectrum Compact Capillary Cartridge, 4-Capillary 36cm |
CE2340 | 1 × 1 each |
Spectrum Compact Cathode Septa Mat |
CE2301 | 1 × 10 each |
Spectrum Compact Cathode Retainer |
CE2302 | 1 × 4 each |
Spectrum Compact Strip Base & Retainer, 32-Well |
CE2332 | 1 × 4 each |
Strip Septa Mat, 8-Well |
CE2308 | 1 × 24 each |
Barcode Scanner |
CE5300 | 1 × 1 each |
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Spectrum Compact Buffer |
CE2300 | 1 × 2 pairs | |
Spectrum Compact Polymer4; use with software v.6138000-XX |
CE2304 | 1 × 4 × 64 wells | |
Spectrum Compact Polymer4; use ONLY with software v.6138200-XX |
CE2404 | 1 × 4 × 96 wells | |
Spectrum Compact Polymer7; use with software v.6138000-XX |
CE2307 | 1 × 4 × 64 wells | |
Spectrum Compact Polymer7; use ONLY with software v.6138200-XX |
CE2407 | 1 × 4 × 96 wells | |
Capillary Preservation Buffer |
CE2399 | 1 × 10ml | |
Spectrum Compact Capillary Cartridge, 4-Capillary 36cm |
CE2340 | 1 × 1 each | |
Spectrum Compact Cathode Septa Mat |
CE2301 | 1 × 10 each | |
Spectrum Compact Cathode Retainer |
CE2302 | 1 × 4 each | |
Spectrum Compact Strip Base & Retainer, 32-Well |
CE2332 | 1 × 4 each | |
Strip Septa Mat, 8-Well |
CE2308 | 1 × 24 each | |
Barcode Scanner |
CE5300 | 1 × 1 each | |
Spectrum Compact CE System Standard Service Agreement |
SA6131 | 1 × 1 each | View Product |
Resources
Articles
Software and Firmware
Related Products
Similar Products
Spectrum Compact CE System Consumables
Plug-and-play prefilled consumables with guided software user interface for the Spectrum Compact CE System.
CE2300, CE2301, CE2302, CE2304, CE2404, CE2307, CE2407, CE2308, CE2332, CE2340, CE2399
Spectrum Compact CE Service and Support Products
Service and warranty products for the Spectrum Compact CE System.
SA6131, SA6132, SA6133, SA6136
Spectrum Compact CE System Qualification Products
Installation Qualification (IQ) and Operational Qualification (OQ) products for the Spectrum Compact CE System.
SA6134, SA6135
Frequently Used With
ProDye® Terminator Sequencing System
An improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix.
CR4324, CR4302, CR4310
GenePrint® 10 System
STR analysis for human cell line authentication.
B9510
GenePrint® 24 System
STR analysis system for mixed sample identification.
B1870, B1874
PowerPlex® 18E System
First 8-color STR multiplex for European forensic laboratories; allows amplification and detection of all 16 ENFSI-recommended loci plus Amelogenin and DYS391 for sex determination.
DC3620, DG5010, DM1601, DG8571
