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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Diamond™ Nucleic Acid Dye

H1181_Diamond--Nucleic-Acid-Dye--500ul_3
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Safe Alternative to Ethidium Bromide

  • Sensitive nucleic acid stain
  • Store at room temperature for quick and easy use without thawing
  • Use with a variety of common gel types and imaging equipment

Size

Catalog number selected: H1181

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This product is discontinued
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Diamond™ Nucleic Acid Dye
500μl
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Diamond™ Nucleic Acid Dye

Diamond™ Nucleic Acid Dye is a sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system, such as by UV transillumination with a Polaroid® or digital camera, GE ImageQuant™ or Bio-Rad Gel Doc™ systems.

The concentrated dye is stable for up to 90 days at room temperature. Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gels. It is much more sensitive than ethidium bromide, so less sample nucleic acid and nucleic acid markers are required for visualization, resulting in increased savings with every gel you run.

Overview of the Diamond™ Nucleic Acid Dye protocol.

Diamond™ Nucleic Acid Dye protocol for staining nucleic acid

Overview of the Diamond™ Nucleic Acid Dye protocol.

Diamond™ Nucleic Acid Dye staining of DNA separated on a 1.2% Clear E-gel®.

Diamond™ Gel Nucleic Acid staining of DNA separated on a 1.2% Clear E-gel®.

Diamond™ Nucleic Acid Dye staining of DNA separated on a 1.2% Clear E-gel®. Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–12: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.

Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.

Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel. Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–10: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size

Diamond™ Nucleic Acid Dye

 H118A 1 × 500μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

Patent Pending.

Resources

Articles

Frequently Used With

BenchTop 1kb DNA Ladder

Thirteen blunt-ended fragments of 250–10,000bp premixed with loading dye.

G7541

Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

V3121, V3125

Wizard® SV Gel and PCR Clean-Up System

Purifies DNA fragments from gel slices or PCR in 15 minutes.

A9281, A9282, A9285, A9283, A9284

x-tracta Gel Extractor

Safely removes agarose slices from gels.

A2121, A2122

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

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