Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

If you are located in the EEAEuropean Economic Area, the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookies in the footer of our website.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

PowerSeq® 46GY System

PS4600_PowerSeq-46GY_3
customize-this-small

Combined Autosomal STR and Y-STR Loci in a Single MPS Library Workflow

  • Targeted panels of familiar loci ensure an expected outcome for data analysis
  • Largest combination of autosomal loci and Y-STR loci in a single kit
  • Improved mixture interpretation results due to better amplicon balance within STRs

Size

Catalog number selected: PS4600

Please Enquire
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
PowerSeq® 46GY System
100 reactions
Your price:

An Optimal MPS Workflow

The PowerSeq® 46GY System contains reagents to amplify autosomal and Y-STR loci as small amplicons (140–300bp) that can be used to prepare MPS libraries and generate sequencing data compatible with Illumina® TruSeq® and MiSeq® technologies. With this workflow, both sequence and allele length polymorphism in the autosomal and Y-STR loci can be identified, thereby increasing the statistical power of inclusion. MPS also eliminates the problem of overlapping alleles completely, allowing us to target more alleles in the multiplex.

The combination of these STR loci and Amelogenin makes this multiplex an effective tool for human identification using MPS, while maintaining compatibility with existing databases worldwide. A combination of familiar markers in the PowerSeq® 46GY System and data analysis software package GeneMarker®HTS from SoftGenetics, LLC, offers a smooth transition to include MPS into your workflow, ensuring that you get the data you want.

Learn more about MPS in this blog post.

Proper bead mixing is critical to successful purification of amplified products. This video illustrates key steps in the purification protocol.

New! This application note provides an alternate protocol for the PowerSeq® 46GY System for use of the KAPA HyperPrep Library Prep Kit for library preparation and ProNex® chemistry for bead purification.

Download PDF

Workflow

The PowerSeq® 46GY System integrates into your laboratory workflow without the burden of analyzing supplementary, unestablished markers—making your transition to MPS a bit easier. When using the PowerSeq® Systems, you can expect to see similar performance with regards to sensitivity, inhibitor tolerance and balance that you've come to expect from our PowerPlex® STR Systems.

powerseq 46gy mps workflow

Balanced Profiles

Good amplicon balance within STRs is important to prevent dropouts, resulting in improved identification of minor contributors in a mixture. Improved balance among loci and the ability to identify minor contributor alleles based on length and sequence polymorphism greatly helps with mixture interpretation.

balanced profiles for powerseq 46gy mps

The PowerSeq® 46GY System generates balanced DNA libraries. Normalized read depth per locus using 1ng 2800M Control DNA (n = 3). To control for sample loading on the MiSeq® instrument, abundance per locus was normalized to the average of total abundance for the sample set. A pool of 96 samples was sequenced simultaneously using MiSeq® Reagent Kit v3, 2 × 300 cycles. Data were analyzed with GeneMarker HTS Software v 2.3 (SoftGenetics, LLC).

Reliable Sensitivity

The PowerSeq® 46GY System offers the same sensitivity that you've come to depend on when using our PowerPlex® systems. While full profiles can be obtained with as little as 62.5pg of input DNA, the balance at low template inputs (100pg) is comparable to the balance seen at the recommended input amount of 1000pg.

powerseq 46gy reliable sensitivity

The PowerSeq® 46GY System generates balanced profiles at low DNA input amounts. Percent of total allele calls per locus at different amounts of 2800M Control DNA (n = 20). A pool of 96 samples was sequenced simultaneously using MiSeq® Reagent Kit v3, 1 × 300 cycles. Data were analyzed with GeneMarker HTS Software v2.2.3 (SoftGenetics, LLC).

 

Large Combination of Dependable Loci

The PowerSeq® 46GY System has the largest combination of autosomal and Y-STR loci in a single kit to generate sequence data and improve your MPS workflow. Amplicons for each locus are designed to be in a range of 140–300bp. The loci included are CODIS core and European Standard Set (ESS) loci, Amelogenin and the Y-STR loci included in the PowerPlex® Y23 System.

Autosomal: CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D1S1656, D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TH01, TPOX, vWA

Y-STR: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, Y-GATA-H4

Other: Amelogenin

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

2800M Control DNA

 DD710A 1 × 25μl 10ng/μl View Product

PowerSeq® 46GY 5X Primer Pair Mix

 DK465B 1 × 500μl

PowerSeq® 5X Master Mix

 DP116A 1 × 500μl

Water, Amplification Grade

 DW099A 2 × 1,250μl View Product

Proteinase K

 V302A 1 × 10mg View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

EE

Resources

Other Resources

Similar Products

PowerPlex® Fusion System

Co-amplification and fluorescent detection of 24 loci.

DC2402, DC2408

PowerPlex® Y23 System

5-dye STR multiplex for amplification of 23 Y-STR loci.

DC2305, DC2320, DM6571

Frequently Used With

PowerSeq® Quant MS System

Quantification of MiSeq® platform compatible MPS libraries.

PS5000

QuantiFluor® ONE dsDNA System

"Add-and-read" quantitation of double-stranded DNA. Includes DNA-binding dye, lambda DNA and buffer.

E4871, E4870

QuantiFluor® dsDNA System

Specific, sensitive dsDNA quantitation system.

E2670, E2671

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.