Spectrum CE System, 8-Capillary

Spectrum CE System

Forensic analysts have a busy schedule filled with processing challenging casework samples and planning urgent, last-minute runs. The Spectrum CE System is a capillary electrophoresis instrument designed to offer more efficient STR analysis and better results, built with input from forensic professionals all over the globe. The result? An instrument that checks all the boxes on your wish list.

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The Spectrum CE System includes a computer and software to have you quickly running analyses.

Flexibility for Your Workflow

You've told us what challenges you face in the lab, and the Spectrum CE System is designed with thoughtful features that give you the freedom to get more done.

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Save Space and Time

Good design means more than just looking pretty on a lab bench; it also means caring about how you interact with Spectrum on a daily basis. Knowing that lab space is often at a premium, we designed the door to open vertically, providing quick access to consumables without knocking things off your bench.

Capacity Where It Counts

Forensic analysis has many challenges; running sample analysis shouldn’t be among them. That’s why Spectrum CE System has capacity for up to four plates of samples, including the ability to run a partial plate. Batching can be a thing of the past.

The Spectrum CE System can run up to four 96-well plates simultaneously.

Smooth Operator

Sometimes you can benefit not just from good hardware choices but also from smart software that gives you a push. Spectrum CE System comes loaded with features to have you quickly running analyses. The software’s ease of use can reduce training time and daily operation effort.

The Spectrum CE System was designed with forensic laboratories in mind to make CE analysis as easy as it gets. An experienced CE user can be up and running on the instrument within an hour. New users will also find the software intuitive and easy to understand.

Spend Less Time Importing and Tracking Samples

Spectrum features automatic recording of plate bar codes and automatic importing of plate map files—making importing sample names a one-click process. There is less opportunity for error with an exact record of the plates analyzed and easier, faster operation.

1. The Spectrum drawer opens to reveal 4 plate positions.

2. Bar-coded plate is loaded into base and retainer.

3. Plate is loaded in an open position (D).

4. Drawer is closed, the bar code is automatically scanned and the plate map file with a matching bar code is identified. The user only needs to select "Link" to automatically import the map into the run queue.

5. Once the plate is linked, the run can be started and plate data will include the identifying bar code information.

Tame Your Most Challenging Samples

Whether you face degraded samples, unseen levels of inhibitors or other kinds of challenges, the Spectrum CE System supports 8-dye technology. Get results you can use, no matter the condition of your samples.

**Amplification of ancient DNA.** DNA was extracted from a petrous bone sample ~800 years old and quantified with the PowerQuant® System. An aliquot was amplified with the PowerPlex® 35GY System and analyzed using the Spectrum CE System. Despite sample degradation, complete genetic information above threshold was obtained from a total of 16 loci plus Amelogenin.

PowerPlex® 18E profile of 0.5ng 2800M Control DNA amplified for 30 cycles in 25μl reactions. Electrophoresis performed on the Spectrum CE System. Data analyzed using GeneMarker® HID Software for Spectrum CE Systems.

High-Quality Results from All Your Samples

We evaluated the performance of the PowerPlex® 35GY System on the Spectrum CE System, including results from a comprehensive concordance study performed in collaboration with the National Institute of Standards and Technology (NIST). Download the brochure.

The PowerPlex® 35GY System allows co-amplification and detection of 35 loci, 24 autosomal, and 11 Y-STR loci (left panel): CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433 and D22S1045, SE33, Penta D, Penta E and Amelogenin; DYS391, DYS385a/b, DYS390, DYS438, DYS448, DYS456, DYS458, DYS481, DYS635 and DYS643. Control amplification of the allelic ladder is also shown (right panel).

Majority of Autosomal STRs are Less Than 325bp

The PowerPlex® 35GY System simultaneously amplifies 35 different loci, including the CODIS 20 core loci, three other highly informative autosomal loci, both Amelogenin and DYS391 for gender confirmation, and ten additional Y-STR loci. The inclusion of Y-STR loci increases the amount of genetic information obtained in a single amplification and can provide valuable data for familial searching. It can also assist in the analysis of sexual assault evidence.

With the two additional colors for detection, there are a total of 15 autosomal STR loci smaller than 250bp, which benefits the analysis of challenging samples.

Amplification of 35 different loci with the PowerPlex 35GY System on the Spectrum CE instrument. — The PowerPlex® 35GY System allows co-amplification and detection of 35 loci, 24 autosomal, and 11 Y-STR loci (represented by lighter shades): CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433 and D22S1045, SE33, Penta D, Penta E and Amelogenin; DYS391, DYS385a/b, DYS390, DYS438, DYS448, DYS456, DYS 458, DYS481, DYS635 and DYS643.

Detect Inhibitors with an IPC

To help you better interpret the quality of your STR results, the PowerPlex® 35GY System is the first PowerPlex® system to include two Quality Indicators, QIS and QIL. The Quality Indicators take the guesswork out of determining if amplification was successful by confirming if DNA is present or if the sample amplification was inhibited. Knowing whether a sample was degraded or inhibited lets you decide whether to repeat sample analysis.

quality indicators — **Quality indicators for the PowerPlex® 35GY System.** Balanced QIS and QIL peaks indicate a successful STR profile (**Panel A**). An STR profile with balanced QIS and QIL peaks but no STR peaks indicates that no template DNA was amplified (**Panel B**). A sloping STR profile with balanced QIS and QIL peaks signals degraded sample (**Panel C**). A sloping STR profile with a low or missing QIL peak suggests the sample is inhibited (**Panel D**).

Compatible with the Kits You Already Love

Spectrum CE System supports 8-dye technology, but if you’re comfortable with existing 5- and 6-color kits that your lab has already validated, we’ve got you covered. You’ll be able to continue using those same products and still take advantage of the increased throughput and flexibility the Spectrum CE System offers.

PowerPlex® Fusion 6C System

2800M Control DNA (500pg) was amplified with PowerPlex® Fusion 6C System. Amplification products were separated using the Spectrum CE System.

GlobalFiler® PCR Amplification Kit

2800M Control DNA (500pg) was amplified with GlobalFiler® PCR Amplification Kit. Amplification products were separated using the Spectrum CE System.

System Consumables and Components

We’ve designed the Spectrum CE System to provide simplified setup and operation. Related consumables, such as prefilled buffer and polymer, are offered in convenient, ready-to-use packaging. Spectrum uses Radio Frequency Identification (RFID) to track key consumables with minimal “hard stops” limited to situations where there is a risk of instrument damage.

Consumables Information

Cat. Number	Description	Size
CE2001	Spectrum Buffer	2 pairs
CE2002	Spectrum Cathode Septa Mat	2-pack
CE2008	Spectrum Capillary Array, 8-Capillary	1 each
CE2012	Spectrum Buffer and Cathode Septa Mat Bundle (CE2001, CE2002)	2 pairs
CE2048	Spectrum Polymer4, 384 Wells	1 each
CE2040	Spectrum Polymer4, 960 Wells	1 each
CE2099	Spectrum Wash Solution	1 each
CE5004	Spectrum Plate Base & Retainer	4 each
CE2696	Septa Mat, 96-Well	10 each

Specifications

Number of Capillaries	8
Capillary Array Length	36cm
Number of Dye Colors	8
Throughput	Up to four 96-well plates
Computer Platform	Windows® 10 PC (provided)
Polymer	Polymer4
Data File Type	.promega, .fsa for fragment analysis
Dimensions	66cm W × 66cm D × 78cm H (26in W × 26in D × 31in H)
Weight	104kg (228lb)
Power Input	100–120/200–240VAC; 6/3A; 50/60Hz

Download Full Specifications Sheet

To learn more about operating and maintaining the instrument, check out our tutorial videos.

Explore Tutorials

GeneMarker®HID Software for Spectrum CE Systems

GeneMarker®HID Software for Spectrum CE Systems

GeneMarker®HID Software for Spectrum CE System (GMHID-Spectrum) supports data analysis with all commercially available human identity kits. A range of reporting options are available to meet LIMS and report printing needs. Analysis and display options can be easily configured to support your laboratory’s workflow. Other benefits include::

Database searching and relationship testing modules
Review tools, such as automated contamination checking and project comparison
Time savings of at least 25%
Less analyst intervention
Supports Spectrum (.promega) and existing CE data (.fsa and .hid files)

Download GeneMarker® HID Software

GeneMarker HID Linked Navigation — Linked Navigation: Simply click on a peak or in the table to highlight data details.

GeneMarker HID multicolor view — Multi-Color View: View/comment/edit all dye channels for an allelic ladder or sample on one screen.

Unparalleled Customer Support

We understand that your work can’t stop, and we’re dedicated to minimizing any interruptions to your lab operations. Our scientists are ready to help, and we expect to be there when you need us.

Service
Expect complete support options, like phone-based and on-site consultation.

Installation
As you transition to include the Spectrum CE System in your lab, we will be with you every step of the way. We offer on-site instrument, software and application training, as well as timely on-site installation and service. These services ensure minimal interruptions to your lab’s operations.

Maintenance
If you want regular service and maintenance for your Spectrum CE System, we can take care of you. In addition to the included, standard 12-month warranty, ongoing Service Agreements are available to keep your Spectrum instrument running smoothly after the warranty period.

Validation Services
We can provide flexible, consultative Validation Services for Spectrum CE System. Our team of knowledgeable former practitioners use their experience and expertise to design a customized validation solution to fit your laboratory’s needs and preferred scope of work.

Protocols

Complete Protocol

Spectrum CE System Operating ManualPDF (18615 KB) – English

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

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Spectrum CE System, 8-Capillary

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