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PowerQuant® System

PQ5002_PowerQuant--System--200rxn_3
DS1221_PowerQuant-Calibration-Kit_3
PQ5002_PowerQuant--System--200rxn_3
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5-Color, 4-Target Probe-Based qPCR Assay

  • Quantifies total amount of amplifiable autosomal and Y-chromosomal DNA in single assay
  • Consistently detects 0.5pg/µl human DNA
  • Detects PCR inhibitors and assesses DNA integrity of your sample

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Catalog number selected: PQ5002

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PowerQuant® System
PowerQuant® System/200 reactions
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Accurate and Sensitive DNA Quantification on the First Try

The PowerQuant® System is a 5-color, 4-target probe-based qPCR assay that quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. This system provides flexible options for 4-point to 7-point standard curve. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.

  • Trust your zero quantification results—zero means zero
  • Obtain reliable quantification results with more consistent auto/Y ratios
  • Maximize your success in STR assays with straightforward DNA quality assessment
  • Shorten time to results with a faster cycling time of approximately 1 hour
  • New software allows sample name import, data analysis and customized visualization

The PowerQuant® System is optimized for use on the following instruments:

  1. Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6)
  2. Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2)
  3. Applied Biosystems® QuantStudio™ 5 Real-Time PCR System (Design and Analysis Desktop Software, Versions 1.5 and 1.5.1)

 

Learn more about how the Casework Direct System and the PowerQuant® System can help improve processing efficiency for sexual assault evidence kits (SAEK).

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Use DNA Quantification Results to Reliably Predict STR Results

The PowerQuant® System can detect DNA at concentrations below which an informative profile can be obtained, even when the maximum sample volume is used in an STR amplification reaction.

Data in the PowerQuant® System table indicate the number of replicates in which the amplification curve crossed the amplification threshold within 39 cycles divided by the total number of replicates tested.
A DNA sample was serially diluted to low template concentrations and quantified in quadruplicate using the PowerQuant® System. Data in the PowerQuant® System table indicate the number of replicates in which the amplification curve crossed the amplification threshold within 39 cycles divided by the total number of replicates tested. Auto = autosomal target, Y = male target, D = degradation target. These samples were also amplified in quadruplicate using the PowerPlex® Fusion System by adding the maximum input volume (15μl) per 25μl amplification reaction (except a template amount of 500ng was added for the 100pg/μl dilution). Data in the PowerPlex® Fusion System table show the average number of alleles detected in the STR reaction when analyzed using an Applied Biosystems® 3130 Genetic Analyzer and a 50RFU threshold. 
Representative PowerPlex® Fusion data obtained with 500pg, 150pg, 15pg and 1.5pg of DNA template.

Representative PowerPlex® Fusion data obtained with DNA template. Casework samples often contain DNA mixtures from multiple persons. The Y-chromosomal target in the PowerQuant® System enables quantification of human male DNA in the sample, helping you evaluate mixtures of male and female DNA. The PowerQuant® System also allows you to make better decisions when faced with a partial or failed STR amplification by differentiating between PCR inhibition and DNA degradation.

Reliably Detect PCR Inhibitors

Reliable detection of inhibitors that can affect DNA quantification and downstream STR assays is a key feature of the PowerQuant® System. The internal PCR control (IPC) has roughly the same level of sensitivity to inhibitors as currently available downstream STR assays, allowing you to determine if a sample is appropriate for amplification or if additional processing is required.

IPC Cq shift predicts downstream STR inhibition.
 

IPC Cq shift predicts downstream STR inhibition

In contrast to the sample with the lower humic acid concentration, the IPC Cq shifts for the samples with higher humic acid (HA) concentration (400ng/µl) indicate the presence of an inhibitor and concomitantly show a significant reduction in STR peak heights in the PowerPlex® Fusion System electropherograms. In this example, the [Auto]/[D] ratios are not affected at these humic acid concentrations. This illustrates that the IPC is more sensitive to inhibitors than the degradation target, enabling distinction between sample inhibition and degradation.

Assess Integrity of Your DNA Sample

The system amplifies a short autosomal and a long autosomal target. After amplification, the PowerQuant® Analysis Software calculates the ratio of DNA concentrations for the shorter and longer amplicons. The resulting degradation ratio helps you determine if a DNA sample is degraded, rather than inhibited, and decide which, if any, STR system is most appropriate for downstream analysis. The experiment below illustrates that the [Auto]/[D] ratio is reflective of sample integrity. 

DNA samples exposed to increasing amounts of UV radiation on the 7500 and QuantStudio 5 Systems

Comparison of [Auto]/[Deg] values obtained from PowerQuant® quantification of degraded DNA sample on the Applied Biosystems® 7500 and QuantStudio™ 5 Real-Time qPCR Systems. Samples were exposed to increasing amounts of UV radiation. 

Want to Automate?

Save time and improve precision by automating your DNA quantification setup. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplifications setup.

Automate your DNA Quantification Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Hardware
Product Cat.#
Four-Position Tube Holder
V1601
Plate Clamp 96
V8251
Plate Stand
V8261

Protocols

Complete Protocol

Quick Protocols

Specifications

You are viewing: PQ5002 Change Configuration

What's in the box?

Item Part # Size Available Separately

PowerQuant® Male gDNA Standard

 DD302A 1 × 150μl View Product

PowerQuant® 20X Primer/Probe/IPC Mix

 DK930A 1 × 220μl

PowerQuant® 2X Master Mix

 DP527A 2 × 1.1ml

Water, Amplification Grade

 DW099A 2 × 1,250μl View Product

PowerQuant® Dilution Buffer

 Y101A 2 × 1.5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: PQ5008 Change Configuration

What's in the box?

Item Part # Size Available Separately

PowerQuant® Male gDNA Standard

 DD302A 3 × 150μl View Product

PowerQuant® 20X Primer/Probe/IPC Mix

 DK930A 4 × 220μl

PowerQuant® 2X Master Mix

 DP527A 8 × 1.1ml

Water, Amplification Grade

 DW099A 5 × 1,250μl View Product

PowerQuant® Dilution Buffer

 Y101A 8 × 1.5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: DS1221 Change Configuration

What's in the box?

Item Part # Size

PowerQuant® Calibration Standard, FAM

 DS106A 1 × 60μl

PowerQuant® Calibration Standard, CFG540

 DS107A 1 × 60μl

PowerQuant® Calibration Standard, TMR

 DS108A 1 × 60μl

PowerQuant® Calibration Standard, Q670

 DS109A 1 × 60μl

PowerQuant® Calibration Standard, CXR

 DS110A 1 × 60μl

PowerQuant® Calibration Buffer

 DS111A 1 × 30ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Resources

Articles

Citations

Software and Firmware

Other Resources

Similar Products

Plexor® HY System

qPCR assay for determining male and total human DNA concentrations.

DC1001, DC1000, DC1500

Frequently Used With

PowerPlex® 18E System

First 8-color STR multiplex for European forensic laboratories; allows amplification and detection of all 16 ENFSI-recommended loci plus Amelogenin and DYS391 for sex determination.

DC3620, DG5010, DM1601, DG8571

PowerPlex® 35GY System

First 8-dye STR multiplex; allows co-amplification and fluorescent detection of the 20 CODIS core loci plus Amelogenin and DYS391 for sex determination.

DC3520, DG5010, DM1551, DG8571

PowerPlex® Fusion 6C System

6-color co-amplification and detection of 27 loci.

DC2705, DC2720, DC2780

PowerPlex® Y23 System

5-dye STR multiplex for amplification of 23 Y-STR loci.

DC2305, DC2320, DM6571

Let's find the product that meets your needs.

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Alessandro

Alessandro

Italy
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