Sexual Assault Evidence Kit (SAEK) Processing
The sheer volume of sexual assault evidentiary samples submitted to forensic laboratories has compelled many laboratories to seek better solutions for processing these challenging samples. We offer reliable solutions that take you through every step in the sexual assault evidence kit (SAEK) workflow, increasing productivity and decreasing turn around time, from sample extraction to analysis.
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Complete SAEK Workflow Solution
We offer instruments, reagents and kits to support every step of the SAEK processing workflow. Our complete workflow starts with effective and consistent Y-screening with the Casework Direct and PowerQuant® Systems. Using samples identified as appropriate for differential extraction, the separation of sperm and non-sperm cells is performed using our Maxwell® DE System on a Maxwell® Instrument. Quantification using the PowerQuant® System followed by STR analysis with PowerPlex® Systems on the Spectrum CE or Spectrum Compact CE Systems.
| Preprocessing and Differential Extraction | DNA Isolation | DNA Quantification | Amplification/STR Analysis |
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| Casework Direct System Rapidly process SAEK and trace DNA samples |
Maxwell® FSC DNA IQ™ Casework Kit Automated benchtop DNA extraction from casework samples |
PowerQuant® System Quantify autosomal and Y-chromosomal DNA and assess sample integrity |
PowerPlex® 35GY System PowerPlex® Y23 System |
| Maxwell® DE System Go from mixed cell samples to ready-to-quantify sperm lysate with a single protocol |
Maxprep® Liquid Handler Complete robotic system for nucleic acid purification |
Plexor® HY System Simultaneously quantify autosomal and Y-chromosome DNA |
Spectrum CE Systems High-throughput capillary electrophoresis systems for forensic and paternity labs |
Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
Why Use a Y-STR System for Your SAEK Workflow?
Y-STR analysis can provide clues in situations where autosomal STR analysis has not yielded sufficient information to help the investigation. For example, excess amounts of female DNA can often make it difficult to detect male DNA when performing autosomal STR analysis. While the autosomal loci included in the PowerPlex® 35GY System help determine the total number of contributors in mixed samples, the 11 Y-STRs help estimate the total male contributors and can detect male DNA from cases where the male perpetrator did not ejaculate, had undergone a vasectomy or where penile penetration did not occur.
Learn more about the PowerPlex® 35GY System.
Infographic: Y Screening vs. Serology
Learn how a "Direct-to-DNA" approach using Y-screening can save time and cost compared to traditional serology methods.
SAEK Workflow Resources
Webinar Series: SAEK Legislation
This webinar series addresses challenges and strategies for approaching SAEK processing. This includes investigations of untested rape kits, effectively managing the workload, and a case study on how Wisconsin is tackling their 6,800+ backlog.
Blog: Forensic Scientists Improve Sexual Assault Kit Turnaround Time with Y-Screening
Learn how Y-screening and the Casework Direct System work together to reduce turnaround time and free up analyst time.
Article: From Backlog to Breakthrough
This article explores three critical strategies to tackle the SAEK backlog: implementation of Y-screening techniques, adoption of automation in forensic processes, and adherence to stringent quality assurance (QA) standards.