Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
1Promega Corporation, 2Tecan Austria GmbH
Publication Date: April 2014
Abstract
Promega has developed reporter bioassays that use activation of gene transcription through NFAT as an early measure of ADCC. These reporter bioassays provide a convenient system for assessing ADCC that uses frozen, thaw-and-use effector and target cells and eliminates the need for cell culture maintenance. Here we describe the assay as performed on the Infinite® 200 PRO Reader with gas control module and find it to be functionally equivalent to using a tissue culture incubator for the reporter induction phase of the ADCC Reporter Bioassay.
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Scientific Style and Format, 7th edition, 2006
Schiro, K., Worzella, T., Cheng, Z., Garvin, D., Surowy, T., Hengstl, T., Fejtl, M. and Oberdanner, C. AN267-Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader. [Internet] April 2014. [cited: year, month, date]. Available from: https://worldwide.promega.com/resources/pubhub/applications-notes/an267-performing-adcc-reporter-bioassay-with-an-environmentally-controlled-microplate-reader/
American Medical Association, Manual of Style, 10th edition, 2007
Schiro, K., Worzella, T., Cheng, Z., Garvin, D., Surowy, T., Hengstl, T., Fejtl, M. and Oberdanner, C. AN267-Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader. Promega Corporation Web site. https://worldwide.promega.com/resources/pubhub/applications-notes/an267-performing-adcc-reporter-bioassay-with-an-environmentally-controlled-microplate-reader/ Updated April 2014. Accessed Month Day, Year.
