Ramos (HiBiT) TCK Bioassay
Simple, Fast and Sensitive Technology to Specifically Measure Target Cell Killing
- Flexible platform to measure the activity of a variety of biologic drugs
- Off-the-shelf Ramos HiBiT Target Cells expressing common immunotherapy targets
- Rapid and convenient workflow
- No signal contribution from effector cells
Ramos (HiBiT) TCK Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JA1411 | Ramos (HiBiT) TCK Bioassay | View Specifications | 1 each | List Price: $ 540.00 Your Price: $ 540.00 | Add to Cart | ||
| JA1415 | Ramos (HiBiT) TCK Bioassay 5X | View Specifications | 1 each | List Price: $ 2,300.00 Your Price: $ 2,300.00 | Add to Cart | ||
| M2472 | Ramos (HiBiT) TCK Bioassay, Propagation Model | View Specifications | 1 each | Please Enquire | |||
| GA6070 | Ramos (HiBiT) Cell Bank | View Specifications | 1 each | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
PBMC ADCC Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3055A20 | Early Access PBMC ADCC Bioassay Kit (Ramos) | View Specifications | 1 each | Please Enquire | |||
| CS3055A22 | Early Access PBMC ADCC Bioassay Kit (Ramos) 5X | View Specifications | 1 each | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
T Cell (CD8+) TDCC Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3055B02 | Early Access Human T Cell (CD8+) TDCC Bioassay (Ramos) | View Specifications | 1 each | Please Enquire | |||
| CS3055B05 | Early Access Human T Cell (CD8+) TDCC Bioassay (Ramos) 5X | View Specifications | 1 each | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Quantify Targeted Cell Killing with Confidence
Principle of the HiBiT TCK Bioassay. Cytotoxic mAbs and/or effector cells are incubated with target cells expressing a HiBiT fusion protein. Upon killing of the target cell, the HiBiT fusion protein is released and binds extracellular LgBiT to create a functional NanoBiT® Luciferase enzyme. Luminescence is measured using a luciferase substrate and the GloMax® Discover System.
Applications
CAR-T Cell-Mediated Cytotoxicity
Ramos (HT-HiBiT) Bioassays are amenable to use in HiBiT TCK Bioassays using CAR-T effector cells. Ramos Cells (HT-HiBiT) were incubated with serially diluted CD19 human CAR-T cells at the indicated E:T ratios. After a 4-, 24-, 48- or 72-hour induction, Bio-Glo-NB™ TCK Reagent was added and luminescence quantified using the GloMax® Discover System. Data were fitted to a four-parameter logistic curve using GraphPad Prism® software. HT = HaloTag, the fusion partner for the HiBiT tag.
Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
HiBiT TCK Bioassays reflect the ADCC MoA of biologic drugs and demonstrate specificity for cell surface antigens. Ramos Cells (HT-HiBiT) were incubated with Human PBMC, ADCC-qualified effector cells in the presence of serial titrations of antibodies, including Control Ab, Anti-CD20 (Cat.# GA1130), as indicated. After a 5-hour induction, Bio-Glo-NB™ TCK Reagent was added and luminescence quantified using the GloMax® Discover System. Data were fitted to a four-parameter logistic curve using GraphPad Prism® software. HT = HaloTag, the fusion partner for the HiBiT tag.
T Cell-Dependent Cell-Mediated Cytotoxicity (TDCC)
HiBiT TCK Bioassays reflect the TDCC MoA of biologic drugs and demonstrate specificity for cell surface antigens. Ramos Cells (HT-HiBiT) were incubated with human T Cells (CD8+) in the presence of bispecific T cell engagers (BiTE®). After a 24-hour induction, Bio-Glo-NB™ TCK Reagent was added and luminescence quantified using the GloMax® Discover System. Data were fitted to a four-parameter logistic curve using GraphPad Prism® software. Data were generated using thaw-and-use cells. HT = HaloTag, the fusion partner for the HiBiT tag.
Data sourced from the Human Protein Atlas transcriptomic data of human cell lines. Version 23.0, updated on 23-06-19 [The Human Protein Atlas].
When Ramos (HT-HiBiT) target cells are cocultured with appropriate effector cells (e.g., CAR-T cells, PBMCs or cytotoxic T cells) they can be specifically used to monitor the activity of biologics directed against receptors expressed on the Ramos Cells (HT-HiBiT), such as CD19, CD20 or CD38. When Ramos Cells (HT-HiBiT) are used in assays with biologics against TAAs they do not express, target cells are not lysed and no increase in luminescent signal is observed. Representative data for different MoA are above.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Fetal Bovine Serum |
J121A | 2 × 4ml | |
RPMI 1640 Medium |
G708A | 3 × 36ml | |
Bio-Glo-NB™ TCK Luciferase Assay Substrate |
JB100A | 1 × 0.2ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101A | 1 × 10ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml | 20μM |
Ramos Cells (HT-HiBiT) |
M246A | 1 × 0.5ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.HaloTag®-HiBiT TCK
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If recipient is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the product, including but not limited to the nucleotide sequence encoding the HiBiT and HaloTag® peptides for which sequences have been stably transfected within the cells. In addition, recipient must use Bio-Glo-NB™ TCK or Bio-Glo-NB™ Lytic Luciferase Assay Systems purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. For uses of HaloTag® technology in this product, recipient must either: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Product cannot be used for proficiency testing.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Fetal Bovine Serum |
J121A | 10 × 4ml | |
RPMI 1640 Medium |
G708A | 15 × 36ml | |
Bio-Glo-NB™ TCK Luciferase Assay Substrate |
JB100A | 5 × 0.2ml | |
Bio-Glo-NB™ TCK Luciferase Assay Buffer |
JB101A | 5 × 10ml | |
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 5 × 0.1ml | 20μM |
Ramos Cells (HT-HiBiT) |
M246A | 5 × 0.5ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.HaloTag®-HiBiT TCK
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If recipient is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the product, including but not limited to the nucleotide sequence encoding the HiBiT and HaloTag® peptides for which sequences have been stably transfected within the cells. In addition, recipient must use Bio-Glo-NB™ TCK or Bio-Glo-NB™ Lytic Luciferase Assay Systems purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. For uses of HaloTag® technology in this product, recipient must either: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Product cannot be used for proficiency testing.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Ramos Cells (HT-HiBiT) (CPM) |
M247A | 2 × 1ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
HaloTag®-HiBiT TCK (CPM)
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If recipient is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the product, including but not limited to the nucleotide sequence encoding the HiBiT and HaloTag® peptides for which sequences have been stably transfected within the cells. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NB™ TCK or Bio-Glo-NB™ Lytic Luciferase Assay Systems purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. For uses of HaloTag® technology in this product, recipient must either: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Product cannot be used for proficiency testing.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Ramos Cells (HT-HiBiT) (CPM) |
M247A | 50 × 1ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
HaloTag®-HiBiT TCK (CPM)
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If recipient is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the product, including but not limited to the nucleotide sequence encoding the HiBiT and HaloTag® peptides for which sequences have been stably transfected within the cells. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NB™ TCK or Bio-Glo-NB™ Lytic Luciferase Assay Systems purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. For uses of HaloTag® technology in this product, recipient must either: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Product cannot be used for proficiency testing.
What's in the box?
| Item | Part # | Size |
|---|---|---|
RPMI 1640 Medium |
G708A | 3 × 36ml |
Fetal Bovine Serum |
J121A | 2 × 4ml |
Nano-Glo® HiBiT Extracellular Buffer |
N243A | 1 × 10ml |
Nano-Glo® HiBiT Extracellular Substrate |
N300A | 1 × 0.2ml |
LgBiT Protein |
N401A | 1 × 0.1ml |
Human PBMC, ADCC-Qualified |
CS3055A15 | 1 × 1.0ml |
PBMC ADCC Bioassay Kit (Ramos), Box 2 |
CS3055A21 | 1 × .5ml |
SDS
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Promega Cell Line Limited Use Label License (LULL)
BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
-
1. Usage and Transfer Restrictions:
- 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
- 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
-
2. Modification Restrictions:
- 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
- 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
-
3. Propagation and Storage:
- 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
-
4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
- 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
- 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
- 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
-
5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
- 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
- 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
- 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
-
6. For uses of HaloTag® Technology in this material, recipient must either:
- 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
- 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
-
7. Disclaimer and Governing Law:
- 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
RPMI 1640 Medium |
G708A | 15 × 36ml |
Fetal Bovine Serum |
J121A | 10 × 4ml |
Nano-Glo® HiBiT Extracellular Buffer |
N243A | 5 × 10ml |
Nano-Glo® HiBiT Extracellular Substrate |
N300A | 5 × 0.2ml |
LgBiT Protein |
N401A | 5 × 0.1ml |
Human PBMC, ADCC-Qualified 5X |
CS3055A18 | 1 × 1 each |
IL-7 Bioassay, Propagation Model |
CS3055A23 | 1 × .5ml |
SDS
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Promega Cell Line Limited Use Label License (LULL)
BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
-
1. Usage and Transfer Restrictions:
- 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
- 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
-
2. Modification Restrictions:
- 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
- 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
-
3. Propagation and Storage:
- 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
-
4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
- 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
- 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
- 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
-
5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
- 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
- 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
- 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
-
6. For uses of HaloTag® Technology in this material, recipient must either:
- 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
- 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
-
7. Disclaimer and Governing Law:
- 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
RPMI 1640 Medium |
G708A | 1 × 36ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
Nano-Glo® HiBiT Extracellular Buffer |
N243A | 1 × 10ml |
Nano-Glo® HiBiT Extracellular Substrate |
N300A | 1 × 0.2ml |
LgBiT Protein |
N401A | 1 × 0.1ml |
Human T Cell (CD8+), TDCC-Qualified |
CS3055B03 | 1 × 1 each |
TDCC Bioassay Kit (Ramos), Box 2 |
CS3055B04 | 1 × 1 each |
SDS
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Promega Cell Line Limited Use Label License (LULL)
BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
-
1. Usage and Transfer Restrictions:
- 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
- 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
-
2. Modification Restrictions:
- 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
- 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
-
3. Propagation and Storage:
- 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
-
4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
- 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
- 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
- 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
-
5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
- 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
- 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
- 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
-
6. For uses of HaloTag® Technology in this material, recipient must either:
- 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
- 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
-
7. Disclaimer and Governing Law:
- 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size |
|---|---|---|
RPMI 1640 Medium |
G708A | 5 × 36ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
Nano-Glo® HiBiT Extracellular Buffer |
N243A | 5 × 10ml |
Nano-Glo® HiBiT Extracellular Substrate |
N300A | 5 × 0.2ml |
LgBiT Protein |
N401A | 5 × 0.1ml |
Human T Cell (CD8+) TDCC-Qualified 5X |
CS3055B06 | 1 × 1 each |
TDCC Bioassay Kit (Ramos), 5X, Box 2 |
CS3055B07 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Promega Cell Line Limited Use Label License (LULL)
BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
-
1. Usage and Transfer Restrictions:
- 1.1. Recipient may use this material for research use only, which includes but is not limited to drug discovery and development, quality assurance testing and product release assays. No transfer, resale or commercial use of this material is allowed without the written consent of Promega Corporation.
- 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including product manufacture, providing a service, information or data to unaffiliated third parties and resale of the material for any use.
-
2. Modification Restrictions:
- 2.1. Recipient shall have no right to modify, derivatize or otherwise create variations of the nucleotide sequence of any proprietary gene, including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes stably transfected within the cells.
- 2.2. Recipient shall have no right to genetically engineer or otherwise modify the cell line.
-
3. Propagation and Storage:
- 3.1. The recipient may not propagate the cells, unless material is marked as Propagation Model, Cell Bank or Master Cell Bank; then recipient may propagate and store the cells for long-term use.
-
4. For all determinations of luminescence or other assay-related activity of this material and its derivatives, recipient must either:
- 4.1. Use the detection systems specified in the protocol or data report and purchased from Promega for all luminescence assays when using this material; or
- 4.2. Contact Promega to obtain a license for use of this material with reagents other than a Bio-Glo™ branded luciferase reagent, including but not limited to:
- 4.2.1 Bio-Glo™, Bio-Glo-NL™, Bio-Glo-NB™ TCK, Bio-Glo-NB™ VLP, Bio-Glo-NB™ Lytic
-
5. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
- 5.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
- 5.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
- 5.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
-
6. For uses of HaloTag® Technology in this material, recipient must either:
- 6.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
- 6.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
-
7. Disclaimer and Governing Law:
- 7.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
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Cell Therapy: Decoding CAR-T
CAR-T cellular immunotherapy is a groundbreaking cancer treatment that uses genetically reprogrammed T-cells to target and destroy cancer cells. Watch this video to learn more about CAR-T cellular immunotherapy and how it is revolutionizing the world of cancer treatment.
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