A Cell-Based Luciferase Reporter Bioassay for Interleukin-2 and Interleukin-15 Testing
Part # PS349
Abstract
1Richard Moravec, 2David Adle, 1Frank Fan and 1Mei Cong
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
2Covance Inc., BioCMC, Greenfield, IN 46140
IL-2 and IL-15 are still clinically important cytokines as researchers look to improve potency, patient tolerance and response by developing new molecules with sustained and targeted activities. PEGylation, superagonists, immunocomplexes, and immunocytokines are current strategies in clinical development.
We have developed a luciferase reporter bioassay which can be used for the quantitation of both IL-2 and IL-15 using the cytokine’s mechanism of action pathway. The bioassay format is based on thaw-and use cells, eliminating the need to establish and pre-culture traditional IL-2 responsive cells such as CTLL-2. This format also provides the benefit of convenience, reproducibility, and transferability. Quantitative measurement of IL-2 or IL-15 using this reporter bioassay is complete in less than 7 hours, versus 2–3 days using traditional proliferation assay protocols. We show the bioassay is stability indicating using heat-stressed aldesleukin samples. And finally the cell line demonstrates stability and specificity.
Printed in USA.