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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

ProFection® Mammalian Transfection System

E1200_ProFection--Mammalian-Transfection-Sys_3
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Calcium Phosphate-Mediated Transfection Method

  • Sufficient reagents for 40 high-efficiency transfections of cells plated in 100mm tissue culture dishes
  • High-efficiency stable or transient transfection of eukaryotic cells

Size

Catalog number selected: E1200

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This product is discontinued
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ProFection® Mammalian Transfection System
40 reactions
Your price:

The introduction of DNA into mammalian cells is facilitated by the Calcium Phosphate-mediated ProFection® Mammalian Transfection System. Calcium phosphate transfection is an effective method for the production of long-term stable transfectants. This method also works well for transient expression of transfected genes and can be used with most adherent cell lines.

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size Concentration

Calcium Chloride

 E109A 1 × 3ml 2M

HEPES 2X Buffered Saline

 E110A 1 × 25ml

Nuclease-Free Water

 E111A 1 × 20ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

Other Resources

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Frequently Used With

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Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.

A2492, A2495, A2496

Beta-Glo® Assay System

Luminescence-based detection of β-Gal activity in mammalian cells.

E4720, E4740, E4780

ONE-Glo™ EX Luciferase Assay System

Improved ONE-Glo™ Assay suitable for most firefly applications. Longer lasting signal than original ONE-Glo™ Assay, with increased stability for flexible use and storage.

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Nano-Glo® Dual-Luciferase Reporter Assay System

Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample.

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