Lumit® hKi-67 Immunoassay for Cell Proliferation

Assess Cell Proliferation in Less Than Two Hours with “Add-Mix-Measure” Assay

Cell proliferation is a fundamental parameter of cell-based research. Investigators currently monitor cell proliferation with methods that can be affected by changes in metabolism, require cumbersome wash steps or use indirect markers of proliferation, radioactive material and specialized equipment.

The Lumit® hKi-67 Immunoassay for Cell Proliferation is an easy add-mix-measure plate-based assay for tracking Ki-67, a well-known marker of cell proliferation, without washing steps or specialized detection equipment. A reader capable of detecting luminescence is the only instrument required.

With this assay, researchers can generate more data with higher confidence and reduce prep work and time to results.

How the Lumit® hKi-67 Immunoassay Works

Primary antibodies to Ki-67 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of Ki-67, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to Ki-67 levels.

Avoid Tedious Wash Steps with Simple Protocol

Illustration of the Lumit® hKi-67 Immunoassay protocol.

Broad Linear Range and Specificity of Lumit® hKi-67 Detection

The broad linear detection range of the Lumit® hKi-67 Immunoassay covers expected Ki-67 levels for most cell models and is specific to Ki-67 detection.

Line graph showing the specificity of the Lumit® hKi-67 Immunoassay detection.

Line graph showing the broad linear range of the Lumit® hKi-67 Immunoassay.

Measure Decreases in Cell Proliferation

Treatment of HCT 116 cells with nutlin-3A did not induce cytotoxicity, as determined using fluorescent CellTox™ Green Assay.

24 hours after treatment with nutlin-3A, Ki-67 expression is reduced in HCT 116, along with reduced viable cell number, which was assessed using the Lumit® hKi-67 Immunoassay and CyQuant™ Direct assay, respectively.

48 hours after treatment with nutlin-3A, Ki-67 expression is reduced in HCT 116, along with reduced viable cell number, which was assessed using the Lumit® hKi-67 Immunoassay and CyQuant™ Direct assay, respectively.

Treatment of HCT 116 cells (10,000/well, 96-well plate) with increasing concentrations of the antiproliferative agent nutlin-3A did not induce cytotoxicity and reduced Ki-67 expression levels in a dose-dependent manner. Ki-67 levels were assessed with the Lumit® hKi-67 Assay, while changes in viable cell number were determined with CyQUANT™ Direct in a parallel assay plate.

Measure Increases in Cell Proliferation

Treatment of CD8+ T cells with IL-2; a CD3/CD28 T Cell activator reveals only modest T cell proliferation.

Treatment of CD8+ T cells with IL-2; a CD3/CD28 T Cell activator reveals significant upregulation of Ki-67, as measured using the Lumit® hKi-67 Immunoassay.

Human CD8+ T cells (StemCell Tech) plated at 80,000 cells per well were treated with CD3/CD28 T cell activator with (orange) and without (blue) 10ng/ml IL-2 for 48 hours. Upregulation of Ki-67 is observed with the Lumit® hKi-67 Immunoassay before T cell proliferation (which begins >72 hours after activation), demonstrating use of this assay as an early indicator of proliferation.

Need to differentiate between antiproliferative effects and cell death?

Quickly and easily assess cell proliferation with the Lumit® hKi-67 Immunoassay for Cell Proliferation, a homogeneous cell-based assay for the detection of hKi-67, a key marker of cell proliferation.

Download the Scientific Poster

Blog Article: Rethinking Cell Proliferation Assays

The innovative Lumit® hKi-67 Immunoassay for Cell Proliferation tracks a marker that is independent of the metabolic state of cells, enabling researchers to be more confident in their assessment of proliferation.

Read About This Study

What's in the box?

Item	Part #	Size
Lumit® Detection Substrate C	CS3076A02	1 × 480μl
Lumit® Immunoassay Buffer C (10X)	CS3076A03	1 × 1.8ml
Lumit® Lysis Buffer II (10X)	CS3076A04	1 × 1.3ml
Human Ki-67 Protein, Partial	CS3076A05	1 × 25μl
Anti-hKi-67 mAb-LgBiT, 400X	CS3076A06	1 × 60μl
Anti-hKi-67 mAb-SmBiT, 400X	CS3076A07	1 × 60μl
CellTox™ Green Dye, 1,000X	G873A	1 × 20μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Promega's Cookie Policy

Lumit® hKi-67 Immunoassay for Cell Proliferation

No-Wash Assay for Ki-67 Detection

Assess Cell Proliferation in Less Than Two Hours with “Add-Mix-Measure” Assay

How the Lumit® hKi-67 Immunoassay Works

Avoid Tedious Wash Steps with Simple Protocol

Broad Linear Range and Specificity of Lumit® hKi-67 Detection

Measure Decreases in Cell Proliferation

Measure Increases in Cell Proliferation

Need to differentiate between antiproliferative effects and cell death?

Blog Article: Rethinking Cell Proliferation Assays

Specifications

What's in the box?

SDS

Certificate of Analysis

Storage Conditions

Patents and Disclaimers

Resources

Articles

Posters

Other Resources

CellTiter-Glo® Luminescent Cell Viability Assay

CellTiter-Glo® 2.0 Cell Viability Assay

RealTime-Glo™ MT Cell Viability Assay

CellTiter 96® AQueous One Solution Cell Proliferation Assay

CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT)

Promega's Cookie Policy

Lumit® hKi-67 Immunoassay for Cell Proliferation

No-Wash Assay for Ki-67 Detection​

Assess Cell Proliferation in Less Than Two Hours with “Add-Mix-Measure” Assay

How the Lumit® hKi-67 Immunoassay Works

Avoid Tedious Wash Steps with Simple Protocol​

Broad Linear Range and Specificity of Lumit® hKi-67 Detection

Measure Decreases in Cell Proliferation

Measure Increases in Cell Proliferation

Need to differentiate between antiproliferative effects and cell death?​

Blog Article: Rethinking Cell Proliferation Assays

Specifications

What's in the box?

SDS

Certificate of Analysis

Storage Conditions

Patents and Disclaimers

Resources

Articles

Posters

Other Resources

Similar Products

CellTiter-Glo® Luminescent Cell Viability Assay

CellTiter-Glo® 2.0 Cell Viability Assay

RealTime-Glo™ MT Cell Viability Assay

CellTiter 96® AQueous One Solution Cell Proliferation Assay

CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT)

Frequently Used With

GloMax® Discover System

MyGlo™ Reagent Reader

Lumit® IFN-γ (Human) Immunoassay

Americas

Pacific Asia

Europe

No-Wash Assay for Ki-67 Detection

Avoid Tedious Wash Steps with Simple Protocol

Need to differentiate between antiproliferative effects and cell death?