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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

ViviRen™ Live Cell Substrate

E6492_ViviRen--Live-Cell-Substrate--3-7mg_3
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Sensitive Quantitation of Renilla Luminescence in Live Cells

  • Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays
  • Permits real-time measurement in multiwell plates
  • High signal-to-background ratios

Size

Catalog number selected: E6491

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ViviRen™ Live Cell Substrate
0.37mg
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Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells

ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.

Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are each supplied as a lyophilized solid.

Assay Advantages

  • Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
  • Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
  • Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
Luminescence reaches near-maximum levels within minutes of ViviRen Live Cell Substrate or coelenterazine addition.

Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition. CHO cells stably expressing Renilla luciferase were exposed to 20µM ViviRen™ Live Cell Substrate or coelenterazine approximately 1.5 minutes after continuous luminescent reading was initiated. For more details, see Figure 5 of TM064.

Protocols

Complete Protocol

Specifications

You are viewing: E6491 Change Configuration

What's in the box?

Item Part # Size

ViviRen™ Live Cell Substrate

 E649A 1 × 0.37mg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: E6492 Change Configuration

What's in the box?

Item Part # Size

ViviRen™ Live Cell Substrate

 E649B 1 × 3.7mg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: E6495 Change Configuration

What's in the box?

Item Part # Size

ViviRen™ Live Cell Substrate

 E649C 1 × 37mg

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Resources

Articles

Citations

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