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Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

FuGENE® 6 Transfection Reagent

FuGENE 6 Transfection Reagent 1ml
FuGENE 6 Transfection Reagent 5 x 1ml
FuGENE 6 Transfection Reagent 0.5ml
FuGENE 6 Transfection Reagent 1ml
customize-this-small

Reliable Transfection for Common Cell Lines

  • Preserve cell viability
  • Excellent compatibility with everyday cell lines
  • Straightforward protocol; minimal optimization

Size

Catalog number selected: E2691

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FuGENE® 6 Transfection Reagent
1ml
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Your Everyday Transfection Reagent

FuGENE® 6 Transfection Reagent provides sturdy results in a wide variety of standard laboratory cell lines, making it ideal for everyday use. The simple protocol eliminates tedious culture medium changes and eliminates a source of variation. The gentle performance of the FuGENE® 6 Transfection Reagent makes it an ideal companion to luciferase reporter gene assays or other sensitive cellular analysis applications.


The Best Choice for Your Everyday Cell Transfections

10446MB-W

Reverse transfection of Jurkat cells with pGL4.13[luc2/SV40] DNA. Four different transfection reagents were used at two different transfection reagent:DNA ratios as indicated below the chart. Three different volumes were used in the experiment also indicated below the chart (X axis). “Luc” represents transfection of pGL4.13[luc2/SV40] DNA and control represents transfection reagent without DNA. Bars indicate relative light units (RLU) as determined by ONE-Glo™ Luciferase Assay System (left axis). Lines indicate % viability (from control with no transfection reagent) as determined by CellTiter-Fluor™ Cell Viability Assay (right axis). Error bars correspond to replicates of four data points.

10181MC-W

Comparison of transfection reagent performance in HEK293 Cells. Cells were transfected with a luciferase reporter plasmid, and luciferase activity was detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. The FuGENE® 6 Transfection Reagent provides high expression levels with minimal impact on cell health.

Simple Protocol Reduces Variables

9729MB-W-a
Plate cells
9729MB-W-b
Mix & Incubate Reagent, Medium and DNA
9729MB-W-c
Add to Cells and Incubate
9729MB-W-d
Measure Results

Cited By Your Peers

If a colleague of yours has published a paper involving transfection, there’s a strong chance she used FuGENE® 6. With tens of thousands of citations, it’s what scientists use when they want a reliable, simple reagent for transfecting common cell lines.

32620075-FuGeneHD_Tool_ProtocolDatabase

Protocols

Complete Protocol

Specifications

You are viewing: E2691 Change Configuration

What's in the box?

Item Part # Size

FuGENE® 6 Transfection Reagent

 E269A 1 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® 6 is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Specifications

You are viewing: E2692 Change Configuration

What's in the box?

Item Part # Size

FuGENE® 6 Transfection Reagent

 E269A 5 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® 6 is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Specifications

You are viewing: E2693 Change Configuration

What's in the box?

Item Part # Size

FuGENE® 6 Transfection Reagent

 E269B 1 × 0.5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® 6 is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Resources

Articles

Citations

Other Resources

Similar Products

FuGENE® HD Transfection Reagent

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E2311, E2312

ViaFect™ Transfection Reagent

High-efficiency, low-toxicity transfection of many cell types, including iPS cells.

E4981, E4982

ProFection® Mammalian Transfection System

Calcium phosphate-based method for stable or transient transfection of eukaryotic cells.

E1200

FuGENE® SI Transfection Reagent

Innovative transfection reagent for the delivery of siRNA, miRNA and other small RNA molecules into a wide range of eukaryotic cell lines.

E9311, E9312

Frequently Used With

Nano-Glo® Dual-Luciferase Reporter Assay System

Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample.

N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551

PureYield™ Plasmid Miniprep System

Up to 15μg transfection-ready plasmid from 3ml cultures in 10 minutes.

A1223, A1222

PureYield™ Plasmid Midiprep System

Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.

A2492, A2495, A2496

ONE-Glo™ EX Luciferase Assay System

Improved ONE-Glo™ Assay suitable for most firefly applications. Longer lasting signal than original ONE-Glo™ Assay, with increased stability for flexible use and storage.

E8110, E8120, E8130, E8150

Let's find the product that meets your needs.

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