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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

FuGENE® SI Transfection Reagent

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e9312-fugene-si-transfection-reagent-5-x-1ml-3
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Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules​

  • High-efficiency transfection in both challenging and routine eukaryotic cell lines​
  • Maximum knockdown with low toxicity—fewer cells required​
  • Simple protocol with minimal optimization​

Size

Catalog number selected: E9311

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FuGENE® SI Transfection Reagent
1ml
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High-Efficiency, Gentle Reagent for Small RNA Delivery​

FuGENE® SI is an innovative, 100% synthetic, multicomponent transfection reagent designed for the delivery of siRNA, miRNA and other small RNA molecules. FuGENE® SI provides high-efficiency delivery of RNA into a wide range of eukaryotic cell lines while providing the gentle, low-toxicity transfection you expect from FuGENE® reagents. Obtain maximum knockdown efficiencies while minimizing impact on cell health, allowing you to get the most out of transfection-based experiments.

Animal-free reagents and strong performance make FuGENE® SI ideal for a variety of RNA transfection applications from functional genomics to siRNA library screens.

The quick and easy protocol allows you to obtain superior results with minimal optimization, freeing-up valuable lab time.

Gentle and Nontoxic siRNA Transfection

Bar graph illustrating that FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection demonstrated using HEK293-GFP cell line.
Bar graph illustrating that FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection demonstrated using NIH3T3-GFP cell line.

FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent. Cells were then analyzed via flow cytometry 48 hours post-transfection to measure total cell viability. Graphs show the gentle and nontoxic transfection of siRNAs using FuGENE® SI.

Superior Knockdown Efficiency

Bar graph illustrating that FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency demonstrated using HEK293-GFP cell line.
Bar graph illustrating that FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency demonstrated using NIH3T3-GFP cell line.

FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent or 0.3µl of Lipofectamine™ RNAiMAX (Life Technologies). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP and total cell viability. Graphs show the superior knockdown performance of FuGENE® SI versus Lipofectamine™ RNAiMAX in HEK293 and NIH3T3 cells.

FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency, as confirmed by Western blot.

FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency, as confirmed by Western blot. Human MDA-MB-468 cells were reverse-transfected in 6-well plates with 40pmol of the indicated targeting siRNAs or negative/positive control (Silencer® Select siRNA, Invitrogen) utilizing either 7.5µl of FuGENE® SI Transfection Reagent or 7.5µl of Lipofectamine™ RNAiMAX per well. The cells were collected 72 hours after transfection and analyzed via Western blot for expression of target genes STAT3 and CEBPB. The Western blot shown here demonstrates the robust, superior knockdown with FuGENE® SI as compared to the market leading competitor.

Efficient Transfection of Labeled Small RNAs

Line graph illustrating that FuGENE® SI provides efficient transfection of labeled small RNAs.

FuGENE® SI provides efficient transfection of labeled small RNAs. A549, COS-7, HEK293 and HeLa cells plated in 96-well microplates were transfected with 0.4µl of FuGENE® SI or a no-reagent negative control and various amounts of FITC-labeled negative control siRNA. Twenty-four hours after transfection, the cells were analyzed via flow cytometry for FITC-positive signal.

Protocols

Complete Protocol

Specifications

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What's in the box?

Item Part # Size

FuGENE® SI Transfection Reagent

 E931A 1 × 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® SI is sold with a Limited Use Label License (LULL).

You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Specifications

You are viewing: E9312 Change Configuration

What's in the box?

Item Part # Size

FuGENE® SI Transfection Reagent

 E931A 5 × 1ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® SI is sold with a Limited Use Label License (LULL).

You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

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