PowerPlex® 35GY System

The First 8-Dye PowerPlex® STR Multiplex System

Rapid cycling conditions for extracted DNA and database samples
Consists of Amelogenin, 23 autosomal STR loci, 11 Y-STR loci and two quality indicators in a single multiplex
Fifteen of the autosomal STR loci are less than 250bp
Optimized for use with the Spectrum CE and Spectrum Compact CE Systems

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PowerPlex® 35GY System 8C Matrix Standard PowerPlex® 35GY QI Reagent CCO ILS 500

Size

200 (or 400 direct-amp) reactions 5 preps 800µl 250µl

Catalog number selected: DC3520

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PowerPlex® 35GY System

PowerPlex® 35GY System/200 (or 400 direct-amp) reactions

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More Information from Your Sample

The PowerPlex® 35GY System is the first 8-dye STR multiplex and allows co-amplification and fluorescent detection of the 20 CODIS core loci plus Amelogenin and DYS391 for sex determination. The inclusion of two additional dye channels relative to previous STR multiplex kits provides improved and more informative analyses for even your most challenging DNA samples.

Fifteen mini-STRs improve results with degraded samples.
System contains the shortest SE33 amplicon of any STR chemistry kit available.
Penta D, Penta E and SE33 loci allow wider database searching.
The PowerPlex® 35GY QI Reagent is required for direct amplification and assists in determining if a sample is degraded or inhibited.

Download PowerPlex® 35GY Brochure

The Most Loci in Any STR Multiplex

PowerPlex® 35GY System loci — **The power of 8 dye channels.** Shifting larger autosomal loci in the PowerPlex® Fusion 6C System into two additional dye channels in the PowerPlex® 35GY System frees up space for Y-STR loci to be included, providing more information from a sample and improving analysis.

One Kit for All Your Sample Types

Designed for amplification of extracted DNA and direct amplification of single-source reference samples, the PowerPlex® 35GY System enables laboratories to process all sample types with one amplification kit. The PowerPlex® 35GY System is optimized to work with 1ng of extracted DNA in a 25μl reaction volume and is compatible with the Spectrum CE System and Spectrum Compact CE System.

Electropherogram of extracted DNA amplified using the PowerPlex® 35GY System. — **Amplifying extracted DNA with the PowerPlex® 35GY System.** The 2800M Control DNA (1ng) was amplified using the PowerPlex® 35GY System. Amplification products were mixed with CCO Internal Lane Standard 500 and separated on the Spectrum CE System. The electropherogram was generated using GeneMarker® HID Software for Spectrum CE Systems. Dye names are shown on the right.

18690ta-b — **Amplifying Extracted DNA with the PowerPlex® 35GY System.** The 2800M Control DNA (1ng) was amplified using the PowerPlex® 35GY System. Amplification products were mixed with CCO Internal Lane Standard 500 and separated on the Spectrum Compact CE System. The electropherogram was generated using GeneMarker® HID Software for Spectrum CE Systems.

As with our 5-dye and 6-dye PowerPlex® Systems, the PowerPlex® 35GY System also supports reduced-volume direct amplification reactions. In one example, DNA from a buccal swab in SwabSolution™ Reagent was amplified for 25 cycles in a 12.5μl reaction volume. Amplified samples were separated on the Spectrum CE System.

Electropherogram of buccal cells direct-amplified using the PowerPlex® 35GY System. — **Direct amplification with the PowerPlex® 35GY System.** An FTA® punch of buccal cells was direct-amplified using the PowerPlex® 35GY System. Amplification products were separated on the Spectrum CE System and analyzed using GeneMarker® HID Software for Spectrum CE Systems.

Quality Indicators Ease Data Interpretation

To help you better interpret the quality of your STR results, the PowerPlex® 35GY System is the first PowerPlex® system to include two Quality Indicators, QIS and QIL. The Quality Indicators take the guesswork out of determining if amplification was successful by confirming if DNA is present or if the sample amplification was inhibited. Knowing whether a sample was degraded or inhibited lets you decide whether to repeat sample analysis.

Electropherogram showing quality indicators for the PowerPlex® 35GY System. — **Quality indicators for the PowerPlex® 35GY System.** Balanced QIS and QIL peaks indicate a successful STR profile (**Panel A**). An STR profile with balanced QIS and QIL peaks but no STR peaks indicates that no template DNA was amplified (**Panel B**). A sloping STR profile with balanced QIS and QIL peaks signals degraded sample (**Panel C**). A sloping STR profile with a low or missing QIL peak suggests the sample is inhibited (**Panel D**).

More Successful Mixture Deconvolution

Mixed casework samples often contain PCR inhibitors, and the minor DNA component may be present at a very low level. The enhanced sensitivity and superior capability to overcome inhibition, as well as inclusion of 11 Y-STR loci in the PowerPlex® 35GY System, enable laboratories to interpret mixed samples more confidently than previously possible.

Electropherogram of extracted DNA from 4-person mixtures amplified using the PowerPlex® 35GY System. — **Better mixture analysis with more Y-STRs in the PowerPlex® 35GY System.** Extracted DNA (1ng) from four-person mixtures was amplified using the PowerPlex® 35GY System. Amplification products were separated on the Spectrum CE System and analyzed using GeneMarker® HID Software for Spectrum CE Systems.

Mini-STRs Improve Success with Difficult Samples

The environmental conditions encountered in forensic casework, mass disasters and missing persons scenarios can lead to severe degradation of human DNA samples. Such highly degraded samples often produce incomplete or no STR profiles due to larger loci that can “drop out”. The inclusion of more loci as mini-STRs in the PowerPlex® 35GY System results in better and more complete profiles, making this system an ideal option to process cold-case samples and newer, difficult sample types.

In the PowerPlex® 35GY System, there are eight common loci with amplicons that are at least 100bp smaller in size than when amplified using the PowerPlex® Fusion 6C System.

Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Protocolos

Complete Protocol

PowerPlex® 35GY System for Use on the Spectrum CE System Technical ManualPDF (4895 KB) – English

PowerPlex® 35GY System for Use on the Spectrum Compact CE System Technical ManualPDF (10812 KB) – English

PowerPlex® Matrix Standards for Use on the Spectrum CE System Technical ManualPDF (3463 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
PowerPlex® 35GY 5X Primer Pair Mix	DK481B	1 × 1ml
PowerPlex® 35GY 5X Master Mix	DP800A	1 × 1ml
Water, Amplification Grade	DW099A	5 × 1,250μl		View Product
CCO Internal Lane Standard 500	DG857A	1 × 250μl
PowerPlex® 35GY Allelic Ladder Mix	DG853B	1 × 100μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación
8C Matrix Mix	DG854A	1 × 150μl
Matrix Dilution Buffer	DM145A	5 × 200μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación
PowerPlex® 35GY QI Reagent	DM155A	1 × 800μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Contenido

Item	Part #	Presentación
CCO Internal Lane Standard 500	DG857A	1 × 250μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Recursos

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PowerPlex® 35GY System

The First 8-Dye PowerPlex® STR Multiplex System

More Information from Your Sample

The Most Loci in Any STR Multiplex

One Kit for All Your Sample Types

2800M Control DNA–Spectrum CE System

2800M Control DNA–Spectrum Compact CE System

Buccal FTA® Punch

Quality Indicators Ease Data Interpretation

At-a-Glance Quality Analysis

More Successful Mixture Deconvolution

M:M:M:F - 4:3:2:1

Mini-STRs Improve Success with Difficult Samples

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Recursos

Software y Firmware

Posters

Otros

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