MyGlo™ Reagent Reader

Streamline Your Assays to Get Results Faster

The MyGlo™ Reagent Reader provides an all-in-one solution to streamline your setup and interpretation of CellTiter-Glo® and other luminescent cell health assays.

Cloud-based, assay-specific apps walk you through every step of your assay on MyGlo, including automated data analysis and visualization.

The Caspase-Glo® 3/7 Application for MyGlo is Now Available. Learn More >

In-App Data Analyses Offer Effortless Insights

MyGlo runs with the ProNect™ Data Platform. Together, they provide a cloud-connected workflow that integrates instrument, assay and data analysis.

Avoid tedious, error-prone manual data analysis and get the insights you need quickly in various formats. For example, when you carry out CellTiter-Glo® Assays with your MyGlo™ Reagent Reader, these data visualizations are automatically generated:

• Cell viability percentage
• Dose response viability curve

• Isolate single compound
• Dose response inhibition curves

See Instrument Sensitivity Data Learn About ProNect™ Data Platform

Image showing examples of data visualizations automatically generated by the MyGlo™ Reagent Reader.

Image of limited-time free sample of CellTiter-Glo® 2.0 Reagent.

Free Sample Included

For a limited time, receive a free 10ml sample of CellTiter-Glo® 2.0 Cell Viability Assay when you purchase a MyGlo™ Reagent Reader.

Carry Out Your Experiments Whenever You Want

Your MyGlo is ready when you are.

Illustration of the MyGlo™ Reagent Reader workflow.

One Time Setup

Create a ProNect™ Data Platform account, install the MyGlo™ setup software on your computer to connect your MyGlo to Wi-Fi and follow the steps in Connect2ProNect for seamless configuration. No waiting for vendor installation, calibration or training.

Compact Footprint

MyGlo is a personal device, the first of its kind for biology laboratories. Its compact footprint—no bigger than a pipette tip box—means you can keep your MyGlo™ Reagent Reader on your bench. No need to share and schedule time on a group instrument.

Analyze Results from Anywhere

The MyGlo™ Reagent Reader's cloud-based user interface gives you the freedom to design experiments, view finished analyses and monitor assay progress from any desktop computer with an internet connection.

Your Go-To Assays Are Now Even Easier

MyGlo™ Reagent Reader and its apps are tailored specifically for select Promega assays and protocols.

CellTiter-Glo® App

The CellTiter-Glo® App is compatible with all versions of our CellTiter-Glo® Cell Viability Assay.

Representation of the CellTiter-Glo® Assay App for MyGlo.

The original CellTiter-Glo® Assay
The highly stable CellTiter-Glo® 2.0 Assay
The CellTiter-Glo® Assay optimized for 3D cell cultures

CellTiter-Glo® Assays

CellTiter-Glo® Assays determine the number of viable cells in culture using bioluminescent technology to quantitate the amount of ATP, an indicator of metabolically active cells.

Cited in over 30,000 publications over 20+ years
Simple, 10-minute, add-mix-measure protocol
Sensitive down to 10 cells per well

Caspase-Glo® 3/7 App

Measure caspase activity with the Caspase-Glo® 3/7 Application—now available on your MyGlo™ Instrument.

The Caspase-Glo® 3/7 App is compatible with the Caspase-Glo® 3/7 Assays.

Representation of the Caspase-Glo® Assay App for MyGlo.

The original Caspase-Glo® 3/7 Assay
The Caspase-Glo® 3/7 3D Assay optimized for 3D cell cultures

Caspase-Glo® 3/7 Assays

The Caspase-Glo® 3/7 Assays detect caspase-3/7 activity of cells in culture to quantitate the amount of luminescence, which is proportional to the amount of caspase activity present, an indicator of apoptosis.

High-sensitivity caspase assays
Simple add-mix-measure protocols
Easily scalable formats

App Specifications and Performance

CellTiter-Glo® Assay

Microplate Types: 96-well, white | Readout Orientation: top | Sensitivity: 10 cells/well

Performance Data

Jurkat cells were seeded in all-white 96-well plates from 10 cells/well to 100,000 cells/well (n=8 replicates). Cell viability was determined by adding an equal volume of CellTiter-Glo® or CellTiter-Glo® 2.0 Reagent to all wells, and the signal was read on a MyGlo™ Reagent Reader. These graphs were generated using Microsoft Excel.

Example Results

Dose response viability curve for Jurkat cells treated with Anti-Fas (human, activating) clone CH11 for 24 hours. Signal was read on a MyGlo™ Reagent Reader.

Dose response inhibition curve for Jurkat cells treated with Anti-Fas (human, activating) clone CH11 for 24 hours. Signal was read on a MyGlo™ Reagent Reader.

Dose response viability curves for Jurkat cells treated with Bortezomib or Palbociclib for 24 hours. Signal was read on a MyGlo™ Reagent Reader.

Dose response inhibition viability curves for Jurkat cells treated with Bortezomib or Palbociclib for 24 hours. Signal was read on a MyGlo™ Reagent Reader.

Jurkat cells were seeded in all-white 96-well plates at 10,000 cells/well and treated with Bortezomib, Palbociclib or Anti-Fas (human, activating) clone CH11 for 24 hours. Data were fit using four-parameter logistic curves. Data were generated using the MyGlo™ Reagent Reader with the ProNect™ Data Platform.

Caspase-Glo® 3/7 Assay

Microplate Types: 96-well, white | Readout Orientation: top | Sensitivity: ~30 apoptotic cells

Performance Data

Jurkat cells were seeded in an all-white 96-well plate from 10 cells/well to 100,000 cells/well and treated with vehicle or 50ng/ml anti-Fas (clone CH11; EMD Millipore Cat.# 05-201) to induce apoptosis (n = 4 replicates per condition). Caspase-3/7 activity was determined by adding an equal volume of Caspase-Glo® 3/7 Reagent to all wells. Assay signal was read on a MyGlo™ Reagent Reader (integration time = 1 second). These graphs were generated using Microsoft Excel.

Example Results

Dose response viability curve for Jurkat cells treated with Anti-Fas (clone CH11) for 4.5 hours. Signal was read on a MyGlo™ Reagent Reader.

Jurkat cells were seeded in an all-white 96-well plate at 10,000 cells/well and treated with increasing concentrations of anti-Fas (clone CH11) for 4.5 hours to induce apoptosis. Subsequently, Caspase-Glo® 3/7 Reagent was added, and data was generated using the MyGlo™ Reagent Reader with the ProNect™ Data Platform. Data were fit using four-parameter logistic regression.

MyGlo isn’t just for measuring cell viability and apoptosis.

More assays and apps are on their way. Your MyGlo™ Reagent Reader will be automatically compatible with new apps—no additional purchase required.

Instrument Specifications

Requirements
Wi-Fi Connectivity	MyGlo™ Reagent Reader requires connection to the internet via a Wi-Fi network. Wi-Fi, WPA2 (i.e., WPA2-PSK or WPA2-Personal), 2.4GHz only. 5GHz, 6GHz, WPA3-Personal networks not supported at this time (see MyGlo™ Setup Instructions).
ProNect™ Data Platform Account	MyGlo™ Reagent Reader is controlled using apps in the ProNect™ Data Platform.
Setup and User Interface	Connect2ProNect: Desktop application for device setup and management runs on Microsoft® Windows 10 or 11. MacOS® and Virtual Machines not supported. ProNect™ Data Platform and Applications: Works with common desktop internet browsers, including Microsoft® Edge® and Google Chrome®. Mobile and tablet browser experiences not supported at this time.
System
Warranty	12 months; see Operating Manual for details.
Regulatory Compliance	For Research Use Only. Not for use in diagnostic procedures.
Weight	570g (1.25lb)
Dimensions	98mm × 140mm × 42mm (W × L × H)
Power Supply	5V
Power Input	2.5W
Environmental
Shipping Temperature	+4°C to +50°C
Operating Temperature	+15°C to +40°C
Operating Relative Humidity	≤80% noncondensing
Operating Altitude	<2,000m
Performance
Linear Dynamic Range	6 logs
Cross Talk	Average <0.02%

Software and Support

ProNect™ Data Platform Apps
Find the apps and resources we've built for your ProNect™ Data Platform account.

Go to ProNect™ Apps

One Call Supports It All
We supply both the reagents and the instrument, so one call to Promega answers any questions you may have.

Contact Technical Service

MyGlo™ Reagent Reader Service Agreement

If you need additional coverage during or after the MyGlo™ Reagent Reader standard warranty period is over, a MyGlo™ Reagent Reader Service Agreement is available. The MyGlo™ Reagent Reader Service Agreement covers all parts, labor and shipping to and from our authorized service locations. This Agreement does not include repair or replacement necessitated by accident, neglect, misuse, unauthorized repair or modification of the instrument.

If a problem arises with the instrument, please contact Promega Technical Service for support. E-mail: techserv@promega.com. If further action is required, you will be given repair options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.

To obtain service during the covered period, please take the following steps:

Contact Promega Technical Services.
Carry out tests as suggested by your Technical Services contact.

If it is determined that further action is required, you will be given options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.

Before returning the instrument, you will be responsible for cleaning it. If the instrument has been exposed to any chemical hazards, consult the Technical Manual for cleaning instructions.

*May not be available in all countries. Contact your local Promega representative for more information.

Protocolos

Complete Protocol

MyGlo™ Reagent Reader Operating ManualPDF (1787 KB) – English

Contenido

Item	Part #	Presentación	Available Separately
MyGlo™ Reagent Reader USB Cable Wall Power Adapter Plug Adapters	MG1000	1 × 1 each
CellTiter-Glo® 2.0 Reagent	G9241	1 × 10ml	View Product

Item

Part #

Presentación

Available Separately

MyGlo™ Reagent Reader

USB Cable
Wall Power Adapter
Plug Adapters

MG1000

1 × 1 each

CellTiter-Glo® 2.0 Reagent

G9241

1 × 10ml

View Product

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Recursos

Artículos

Software y Firmware

Otros

Productos Similares

GloMax® Navigator System

Luminómetro para microplacas.

GM2000, GM2010

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MyGlo™ Reagent Reader

Simplify Setup and Analysis of Cell Health Assays with a Personal 96-Well Device Powered by the ProNect™ Data Platform

Streamline Your Assays to Get Results Faster

In-App Data Analyses Offer Effortless Insights

Free Sample Included

Carry Out Your Experiments Whenever You Want

Your MyGlo is ready when you are.

One Time Setup

Compact Footprint

Analyze Results from Anywhere

Your Go-To Assays Are Now Even Easier

MyGlo™ Reagent Reader and its apps are tailored specifically for select Promega assays and protocols.

CellTiter-Glo® App

CellTiter-Glo® Assays

Caspase-Glo® 3/7 App

Caspase-Glo® 3/7 Assays

App Specifications and Performance

CellTiter-Glo® Assay

Performance Data

Caspase-Glo® 3/7 Assay

Performance Data

MyGlo isn’t just for measuring cell viability and apoptosis.

Instrument Specifications

Software and Support

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Especificaciones

SDS

Certificado de Análisis

Recursos

Artículos

Software y Firmware

Otros

Productos Similares

GloMax® Navigator System

Usado con frecuencia junto con

CellTiter-Glo® 2.0 Cell Viability Assay

CellTiter-Glo® One Solution Assay

CellTiter-Glo® Luminescent Cell Viability Assay

CellTiter-Glo® 3D Cell Viability Assay

Caspase-Glo® 3/7 Assay System

Caspase-Glo® 3/7 3D Assay

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