Lumit® IL-6 (Human) Immunoassay

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® IL-6 Immunoassay quantitatively measures released IL-6 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® IL-6 Immunoassay Works

Primary antibodies to IL-6 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-6, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-6 levels.

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® IL-6 Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

Assay	IL-6 Human Immunoassay
Limit of Detection (LOD)	7.5pg/ml (3 SD above background)
Dynamic Range	18.2pg/ml – 25ng/ml
Minimal Detectable Dose (MDD)	5.0pg/ml (2 SD above background)
Assay Time	70 minutes
Sample Type	Cell culture supernatants

SD=Standard Deviation

Rapid Detection of IL-6 Release from PBMCs

Comparison of IL-6 release from PBMCs using Lumit IL-6 Human Immunoassay and standard ELISA.17953ma-w — The Lumit® IL-6 (Human) Immunoassay (left) and standard ELISA (right) were used to quantify IL-6 release from PBMCs treated with three inducers (cell stimulation cocktail, lipopolysaccharide or R848) for up to 72 hours. The results are similar, while the Lumit® Immunoassay was completed in one fifth the time of the ELISA, with no transfer and wash steps or plate washer needed.

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

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For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocolos

Complete Protocol

Lumit™ IL-6 (Human) Immunoassay Technical ManualPDF (1925 KB) – English

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405A	1 × 160μl
Lumit® Detection Buffer B	VB406A	1 × 3.2ml
Human IL-6 Standard	W128A	1 × 25μl	10μg/ml
Anti-hIL-6 mAb-SmBiT, 1000X	W129A	1 × 15μl
Anti-hIL-6 mAb-LgBiT, 1000X	W130A	1 × 15μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405A	5 × 160μl
Lumit® Detection Buffer B	VB406A	5 × 3.2ml
Human IL-6 Standard	W128A	1 × 25μl	10μg/ml
Anti-hIL-6 mAb-SmBiT, 1000X	W129A	5 × 15μl
Anti-hIL-6 mAb-LgBiT, 1000X	W130A	5 × 15μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405B	1 × 1.25ml
Lumit® Detection Buffer B	VB406B	1 × 25ml
Human IL-6 Standard	W128A	1 × 25μl	10μg/ml
Anti-hIL-6 mAb-SmBiT, 1000X	W129B	1 × 150μl
Anti-hIL-6 mAb-LgBiT, 1000X	W130B	1 × 150μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Recursos

Artículos

Posters

Homogeneous, Bioluminescent Cytokine Assay Applied to a Monocyte Activation Test for Pyrogen Detection

Otros

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Lumit® IL-6 (Human) Immunoassay

No-Wash Immunoassay for IL-6 Detection

Quantify Cytokine Release Without Tedious Wash Steps

How the Lumit® IL-6 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® IL-6 Detection

Rapid Detection of IL-6 Release from PBMCs

Explore Additional Cytokine Detection Assays

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Posters

Otros

Productos Similares

Lumit® IL-1β Human/Mouse Immunoassay

Lumit® Active IL-18 (Human) Immunoassay

Lumit® Immunoassay Labeling Kit and Detection Reagents

Lumit® IL-2 (Human) Immunoassay

Lumit® IL-4 (Human) Immunoassay

Usado con frecuencia junto con

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LDH-Glo™ Cytotoxicity Assay

T Cell Activation Bioassay

GloMax® Discover System

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