IdeS Protease and IdeZ Protease

IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading proteases that cleave with high specificity.

IdeS Protease is derived from Streptococcus pyogenes. It is an engineered, recombinant protease overexpressed in E. coli. IdeS Protease cleaves IgG at a single site below the hinge region, yielding F(ab')₂ and Fc fragments as shown here.

IdeZ Protease is derived from Streptococcus equi subspecies zooepidemicus. It is also an engineered, recombinant protease overexpressed in E. coli. Like IdeS, IdeZ Protease specifically cleaves IgG molecules below the hinge region, yielding F(ab')₂ and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.