Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction

Part # PS328

Abstract

Terry Riss, Sarah Duellman, Brad Hook, Mark Bratz, Samantha Lewis
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711 

Although the implementation of 3D cell culture models is growing rapidly, there is an unmet need to establish in vitro assay methods to interrogate larger and more complex 3D structures. We report here the results of using a novel small molecule probe that viable cells convert into a substrate for a shrimp-derived luciferase to generate a signal proportional to the number of viable cells. The detection reagent can be incubated with cells and produce a luminescent signal for days while having minimal effect on the biology of the system.

Printed in USA.