HaloTag® Fluorescent Ligands
Covalently Attach Fluorescent Labels or Affinity Tags to Membrane or Intracellular Proteins
- Cell-permeant TMR, Oregon Green®, diAcFAM and Coumarin ligands readily cross the cell membrane for labeling intracellular proteins
- Cell-impermeant tags, Alexa Fluor® 488 and 660, are now available in the new lyophilized format
- Lyophilized ligands are soluble in cell media or aqueous buffers
Imaging Ligands
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3495B06 | Early Access Lyophilized HaloTag® Alexa Fluor® 488 Ligand | View Specifications | 1 each | Consultar Precio | |||
| CS3495B08 | Early Access Lyophilized HaloTag® Alexa Fluor® 660 Ligand | View Specifications | 1 each | Consultar Precio | |||
| G8251 | HaloTag® TMR Ligand | View Specifications | 30μl | List Price: $ 692.00 Your price: $ 692.00 | Add to Cart | ||
| G8252 | HaloTag® TMR Ligand | View Specifications | 15μl | List Price: $ 428.00 Your price: $ 428.00 | Add to Cart | ||
| G2801 | HaloTag® Oregon Green® Ligand | View Specifications | 30μl | List Price: $ 668.00 Your price: $ 668.00 | Add to Cart | ||
| G2802 | HaloTag® Oregon Green® Ligand | View Specifications | 15μl | List Price: $ 416.00 Your price: $ 416.00 | Add to Cart | ||
| G8272 | HaloTag® diAcFAM Ligand | View Specifications | 30μl | List Price: $ 672.00 Your price: $ 672.00 | Add to Cart | ||
| G8273 | HaloTag® diAcFAM Ligand | View Specifications | 15μl | List Price: $ 428.00 Your price: $ 428.00 | Add to Cart | ||
| G8581 | HaloTag® Coumarin Ligand | View Specifications | 30μl | List Price: $ 699.00 Your price: $ 699.00 | Add to Cart | ||
| G8582 | HaloTag® Coumarin Ligand | View Specifications | 15μl | List Price: $ 433.00 Your price: $ 433.00 | Add to Cart | ||
| G1001 | HaloTag® Alexa Fluor® 488 Ligand | View Specifications | 30μl | List Price: $ 681.00 Your price: $ 681.00 | Add to Cart | ||
| G1002 | HaloTag® Alexa Fluor® 488 Ligand | View Specifications | 15μl | List Price: $ 416.00 Your price: $ 416.00 | Add to Cart | ||
| G8471 | HaloTag® Alexa Fluor® 660 Ligand | View Specifications | 30μl | List Price: $ 699.00 Your price: $ 699.00 | Add to Cart | ||
| G8472 | HaloTag® Alexa Fluor® 660 Ligand | View Specifications | 15μl | List Price: $ 428.00 Your price: $ 428.00 | Add to Cart | ||
| G2991 | HaloTag® TMRDirect™ Ligand | View Specifications | 30μl | List Price: $ 722.00 Your price: $ 722.00 | Add to Cart | ||
| G3221 | HaloTag® R110Direct™ Ligand | View Specifications | 30μl | List Price: $ 722.00 Your price: $ 722.00 | Add to Cart |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Affinity Tags for Protein Purification
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| G8281 | HaloTag® Biotin Ligand | View Specifications | 30μl | List Price: $ 715.00 Your price: $ 715.00 | Add to Cart | ||
| G8282 | HaloTag® Biotin Ligand | View Specifications | 15μl | List Price: $ 428.00 Your price: $ 428.00 | Add to Cart | ||
| G8591 | HaloTag® PEG-Biotin Ligand | View Specifications | 30μl | List Price: $ 692.00 Your price: $ 692.00 | Add to Cart | ||
| G8592 | HaloTag® PEG-Biotin Ligand | View Specifications | 15μl | List Price: $ 433.00 Your price: $ 433.00 | Add to Cart |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
HaloTag® Ligands
The HaloTag® technology portfolio offers ligands with a variety of functionalities, including fluorescent labels, affinity tags and solid-phase-attachment abilities. The ligands rapidly form covalent bonds under general physiological conditions; the bonds are highly specific and essentially irreversible. HaloTag® Fluorescent Ligands enable you to add the chloroalkane group, with which HaloTag® protein reacts, to any compound or surface with a compatible chemical group. The possible applications are endless.
Applications
- Pull Down Protein Complexes: The spacer and reactive linker of the HaloTag® PEG-Biotin Ligand provide ideal pull-down capabilities. Alternatively, pull down directly with the HaloLink™ Resin.
- Image Fixed Cells: The covalent bond is stable, allowing imaging of fixed cells and analysis of the labeled protein under stringent conditions.
- Introduce Novel Functionalities or Perform Sequential Labeling: The open architecture of the technology enables the use of different ligands for multiple applications.
- Design Only One Genetic Construct for Multiple Experiments: Obtain new functionality by using a different HaloTag® Ligand without having to design and clone a new expression construct.
- Analyze Labeled Fusion Proteins Using SDS-PAGE, Mass Spectrometry, etc.: The bound ligand is stable under denaturing conditions.
For more information, see the Protocols & Applications Guide.
HaloTag® Technology Overview
HaloTag® Fluorescent Ligands for Cellular Imaging
We offer a variety of cell-permeable and cell-impermeable ligands for cellular imaging. Cell-permeable ligands readily cross the cell membrane and, therefore, can be used to label intracellular proteins. Cell-impermeant ligands, which cannot cross the cell membrane, can be used to label cell surface proteins.
Cell-permeant ligands (rapid-labeling protocol):
- HaloTag® TMR Ligand (555Ex/585Em)
- HaloTag® Oregon Green® Ligand (496Ex/516Em)
- HaloTag® diAcFAM Ligand (494Ex/526Em)
- HaloTag® Coumarin Ligand (353Ex/434Em)
Cell-permeant ligands ("no-wash" protocol):
-
HaloTag® TMRDirect™ Ligand (555Ex/585Em)
-
HaloTag® R110Direct™ Ligand (502Ex/527Em)
Cell-impermeant ligands for cell-surface labeling (rapid-labeling protocol):
- HaloTag® Alexa Fluor® 488 Ligand (494Ex/517Em)
- HaloTag® Alexa Fluor® 660 Ligand (663Ex/690Em)
Live-cell labeling with the HaloTag® Technology. Schematics show live-cell labeling options. Panel A. Rapid labeling. Panel B. No-wash labeling.
Benefits of Lyophilized HaloTag® Ligands
We offer select HaloTag® Ligands in a new, DMSO-free format designed to enhance your workflow while minimizing waste. The lyophilized format provides the following benefits:
- Streamlined Workflow: Directly resuspend the ligand in aqueous solutions, including cell media.
- Elimination of Organic Solvents: There's no longer a requirement for organic solvents that might interfere with your experiments.
- Precise Quantification: Each vial contains a consistent quantity of lyophilized ligand.
- Transparent Packaging: The clear vials allow easy visualization of the ligand, ensuring accuracy and confidence in your preparations.
- Individual Aliquots: Premade aliquots reduce waste and improve efficiency when conducting multiple experiments.
Live cell labeling with impermeable HaloTag® Alexa Fluor® 488 and HaloTag® Alexa Fluor® 660 Ligands. U2OS cells stably expressing HaloTag® protein fused to the truncated beta 1 integrin receptor extracellular surface sequence or control U2OS cells expressing HaloTag® protein fused with three copies of a nuclear localization sequence were adhered to glass-bottom 96-well plates and labeled with Alexa Fluor® 488 or Alexa Fluor® 660 Ligand for 30 minutes at 37°C + CO2 in a cell culture incubator. Cell medium was replaced with a phenol-red-free medium. Cells were imaged with 488nm laser excitation for Alexa Fluor® 488 Ligand (Panel A) and 640nm laser excitation for Alexa Fluor® 660 Ligand (Panel B). In HaloTag-expressing cells, labeling was restricted to the membrane. Control U2OS cells expressing HaloTag in the nucleus showed no labeling. Images were collected using a Nikon AX/AXR confocal microscope with a 20X objective.
Protocols
Complete Protocol
Protocolos Rápidos
Protocolos en Vídeo
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Lyophilized HaloTag® Alexa Fluor® 488 Ligand |
CS3495B05 | 5 × 1 each |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to
- (1) use in further material manufacture; and
- (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Lyophilized HaloTag® Alexa Fluor® 660 Ligand |
CS3495B07 | 5 × 1 each |
Limited Use Label License
BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
Researcher may use this material for research use only; no transfer, or commercial use of this material is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to
- (1) use in further material manufacture; and
- (2) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations or derivatives of the material. No other use of this material is authorized without the prior express written consent of Promega. Notwithstanding the foregoing, researcher may use this material in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the material. No other use of this material is authorized without the prior express written consent of Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® TMR Ligand |
G825A | 1 × 30μl | 5mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® TMR Ligand |
G825B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® Oregon Green® Ligand |
G280A | 1 × 30μl | 1mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® Oregon Green® Ligand |
G280B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® diAcFAM Ligand |
G827B | 1 × 30μl | 1mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® diAcFAM Ligand |
G827C | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® Coumarin Ligand |
G858A | 1 × 30μl | 10mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® Coumarin Ligand |
G858B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® Alexa Fluor® 488 Ligand |
G100A | 1 × 30μl | 1mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® Alexa Fluor® 488 Ligand |
G100B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
| HaloTag® Alexa Fluor® 660 Ligand | G847A | 1 × 30μl | 3.5mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
| HaloTag® Alexa Fluor® 660 Ligand | G847B | 1 × 15μl | 3.5mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
HaloTag® TMRDirect™ Ligand |
G299A | 1 × 30μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® R110Direct™ Ligand |
G322A | 1 × 30μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® Biotin Ligand |
G828A | 1 × 30μl | 5mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® Biotin Ligand |
G828B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
HaloTag® PEG-Biotin Ligand |
G859A | 1 × 30μl | 5mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
HaloTag® PEG-Biotin Ligand |
G859B | 1 × 15μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
Resources
Artículos
- Human Flexi® ORF Clones Ready for HaloTag® Labeling
- Expression of Fusion Proteins: How to Get Started with the HaloTag® Technology
- HaloTag® for High Protein Yield and Purity
- Image Live and Fixed Cells with Multicolor Interchangeable Fluorescent Labels
- Simple and Reliable Labeling from Single Wells to High Content Screens
- Analyzing the Human Proteasome with Proteomics Techniques
- Protein Labeling, Single Well to High-Content Screens
- Cell Surface HaloTag® Technology Spatial Separation and Bidirectional Trafficking of Proteins
- HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells
- HaloTag™ Interchangeable Labeling Technology for Cell Imaging Protein Capture and Immobilization
Related Products
Productos Similares
Janelia Fluor® HaloTag® Ligands
Dyes para estudios de marcaje fluorescente, imagen confocal y estudios de high-resolution single-molecule imaging de proteínas de fusión HaloTag® en células vivas.
HT1110, HT1100, HT1010, CS315102, GA1110, GA1111, HT1020, HT1040, HT1050, GA1120, GA1121, HT1060, HT1030, HT1070, CS3495B02, CS3495B04
HaloTag® Protein Purification System
Expresión y solubilidad mejoradas de proteínas recombinantes con ligandos HaloTag®.
G6280, G6270
HaloLink™ Resin
Ligando HaloTag® unido a beads de sefarosa que une de forma específica y rápida proteínas de fusión HaloTag®.
G1912, G1913, G1914, G1915
HaloTag® Ligand Building Blocks
Permite utilizar cualquier reporter, proteína o ácido nucleico que contenga grupos funcionales específicos con la proteína HaloTag®.
P6711, P6741, P1691, P6751
Usado con frecuencia junto con
HaloTag® Mammalian Pull-Down Systems
Sistemas que permiten la captura rápida de pares de proteínas simples o complejos de forma directa a partir de lisados, además de pasar de pull-downs a estudios de imaging utilizando el mismo constructo.
G6509, G6500, G6504, G6591
HaloTag® Mammalian Protein Purification System
Permite obtener proteína recombinante de alta pureza procedente de cultivos celulares de mamíferos, incluso con proteínas expresadas a bajos niveles.
G6790, G6795
ProTEV Plus
Proteasa diseñada por ingeniería genética con mayor estabilidad y una actividad más prolongada que la proteasa TEV nativa.
V6101, V6102
