Diamond™ Nucleic Acid Dye

Diamond™ Nucleic Acid Dye is a sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system, such as by UV transillumination with a Polaroid® or digital camera, GE ImageQuant™ or Bio-Rad Gel Doc™ systems.

The concentrated dye is stable for up to 90 days at room temperature. Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gels. It is much more sensitive than ethidium bromide, so less sample nucleic acid and nucleic acid markers are required for visualization, resulting in increased savings with every gel you run.

Diamond™ Nucleic Acid Dye protocol for staining nucleic acid — **Overview of the Diamond™ Nucleic Acid Dye protocol.**

Diamond™ Gel Nucleic Acid staining of DNA separated on a 1.2% Clear E-gel®. — **Diamond™ Nucleic Acid Dye staining of DNA separated on a 1.2% Clear E-gel®.** Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–12: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

**Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.** Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–10: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

Protocolos

Complete Protocol

Diamond™ Nucleic Acid Dye Technical ManualPDF (1366 KB) – English

Protocolos Rápidos

Diamond Nucleic Acid Dye FB142PDF (97 KB) – English

Contenido

Item	Part #	Presentación
Diamond™ Nucleic Acid Dye	H118A	1 × 500μl

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Store at room temperature (22–25°C) for up to 90 days. Store at –20°C for long-term storage.

Patentes y Exclusiones

Patent Pending.

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Diamond™ Nucleic Acid Dye

Safe Alternative to Ethidium Bromide